| 摘要: |
| 【目的】揭示SIT转运蛋白家族在焦枯病菌铁元素摄入机制,以及铁载体 铁转运蛋白在焦枯病菌侵染桉树过程中所扮演的角色。【方法】 采用HMMER软件对焦枯病菌SIT转运蛋白进行鉴定，并用InterPro、TMHMM、TCDB数据库等的在线服务对焦枯病菌SIT转运蛋白的结构域、跨膜螺旋和功能进行预测，用MEGA7.0软件进行多菌种比较分析，最后采用qPCR对焦枯病菌侵染桉树48 h时SIT基因的转录表达情况进行验证。【结果】桉树焦枯病菌共含有16个SIT转运蛋白，约占总编码蛋白的0.11%，主要分为铁 铁载体转运蛋白、铁载体-铁:H+同向转运蛋白和铁载体-铁转运蛋白；亚细胞定位预测结果显示，除预测CpSit13转运蛋白位于内质网上外，其余均在细胞膜上。桉树焦枯病菌SIT转运蛋白的序列长度为400~603 aa，其结构域均为MFS结构域，跨膜螺旋为10~14个。基因表达谱分析显示，有10个SIT基因上调表达，有2个下调表达，有4个表达不显著。其中CpSit1、CpSit5、CpSit8、CpSit14和CpSit16相对分支中其他基因具有较高的转录量。qPCR验证结果与基因表达谱具有较高的一致性。【结论】CpSit1、CpSit5、CpSit8、CpSit14和CpSit16基因在焦枯病菌侵染桉树过程中具有较为重要的作用。 |
| 关键词: 桉树 焦枯病菌 SIT蛋白 生物信息学 转录表达 |
| DOI： |
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| 基金项目:福建省财政厅项目（K81139238，K8113001A）；福建农林大学科技创新专项（KFA17053A） |
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| Identification and expression of Siderophore-iron transporter in Calonectria pseudoreteaudii |
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LIU Hongyi,CHEN Huijie,LI Huimin,et al
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| Abstract: |
| 【Objective】The objective of this study is to reveal the role of SIT transporters in the Calonectria pseudoreteaudii iron uptake mechanism and that of siderophore iron transporter in the process of infecting the Eucalyptus.【Method】The SIT transporters of Calonectria pseudoreteaudii were identified by HMMER software.The domain,transmembrane region and function of SIT transporter were predicted by InterPro,TMHMM,TCDB database and other online services.Then,MEGA7.0 was used to compare Calonectria pseudoreteudii with other fungal species.Finally,qPCR was used to confirm the transcript expression of SIT gene 48 h after infecting Eucalyptus.【Result】 The Calonectria pseudoreteaudii had 16 SIT transporters, including ferri siderophore transporter,siderophore-iron:H+ symporter and siderophore-iron transporter.They accounted for 0.11% of the total coding proteins.The prediction of subcellular location showed that almost all of the SIT transporters were located in plasma membrane,except for CpSit13,which was located in endoplasmic reticulum.The protein lengths of SIT transporters ranged from 400 to 603 aa,and the domain of these SIT transporters was MFS domain with the transmembrane helix of 10 to 14.The digital gene expression showed that 10 SIT genes were up regulated,2 SIT genes were down regulated and 4 SIT genes were not significantly expressed.Among them,the relative transcription expression levels of CpSit1,CpSit5,CpSit8,CpSit14 and CpSit16 were higher than other SIT genes in the same branch.The qPCR showed high similarity with the digital gene expression.【Conclusion】CpSit1,CpSit5,CpSit8,CpSit14 and CpSit16 may play an important role during the process of infecting Eucalyptus by Calonectria pseudoreteudii. |
| Key words: Eucalyptus Calonectria pseudoreteaudii SIT protein bioinformatics transcription expression |
