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陕甘地区猪PCV3流行毒株的检测
朱小甫1, 吴旭锦1
咸阳职业技术学院 畜牧兽医研究所
摘要:
【目的】调查陕甘地区猪场PCV3流行情况,为进一步研究与防控PCV3奠定基础。【方法】根据GenBank中PCV3基因序列,设计并合成了1对引物,对96个猪场的96份混合样本进行PCV3检测,将阳性样品进行序列测定分析,同时对PCV3阳性样品进行6种常见猪群病毒病检测。【结果】检测出了PCV3阳性样品8份,阳性率为8.33%。其中7份样品与其他6种常见猪群病毒存在不同程度的混合感染现象,混合感染率87.5%。序列分析表明,陕甘地区猪场8株PCV3基因片段核苷酸同源性在98.3%~100%,与不同地域参考毒株的同源性在97.6%~100%。【结论】陕甘地区2015年样品中即存在PCV3感染,不同地区的PCV3基因同源性较高,遗传稳定;基因进化树显示不同地域PCV3毒株交叉分布,没有明显的地域特征。
关键词:  猪圆环病毒3型  陕甘地区  序列分析  流行病学
DOI:
分类号:
基金项目:陕西省科技计划项目(2017NY-097)
Detection of PCV3 prevalent strains in pigs in Shaanxi and Gansu
ZHU Xiaofu,WU Xujin
Abstract:
【Objective】This paper investigated the prevalence of PCV3 in pig farms in Shaanxi and Gansu to provide basis for further research as well as prevention and control of PCV3.【Method】According to the sequence of PCV3 gene in GenBank,a pair of primers were designed and synthesized,and PCV3 was detected in 96 pig farms samples.The positive samples were sequenced and analyzed,and six swine herd virus tests were also performed on PCV3 positive samples. 【Result】Eight PCV3 positive samples were obtained with the positive rate of 8.33%.Among them,7 were mixed with six swine herd viruses,and the mixed infection rate was 87.5%.Sequence analysis showed that the homology of 8 PCV3 gene fragments in Shaanxi and Gansu pig farms was 98.3%-100%,and the homology with reference strains in different regions was 97.6%-100%.【Conclusion】There was PCV3 infection in Shaanxi and Gansu in 2015 with high homology among different regions and stable genetic inheritance.Phylogenetic tree showed that the PCV3 strains were cross distributed in different regions without significant regional characteristics.
Key words:  porcine circovirus type 3  Shaanxi Gansu region  sequence analysis  epidemiology