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一株氧化木糖无色杆菌对Cd的固定作用
李 哲1, 吴 迪1, 张秀芳,等1
吉林农业大学 资源与环境学院
摘要:
【目的】研究一株氧化木糖无色杆菌LAX2对镉(Cd)的碳酸盐矿化作用及其对Cd污染稻田土壤的修复效果。【方法】将LAX2接种于含0~40 mg/L Cd2+的牛肉膏蛋白胨液体培养基中,测定LAX2对Cd的耐受性。在LAX2发酵液中加入0.1 mol/L CdCl2放置12 h,利用X-射线衍射、红外光谱、扫描电镜和能谱对菌株LAX2矿化固结Cd的产物特征进行分析,并通过水稻盆栽试验验证菌株LAX2对Cd污染土壤的修复效果。【结果】菌株LAX2对Cd2+的耐受能力较强,当溶液中Cd2+质量浓度达35 mg/L时,其生长不受明显影响;但当Cd2+质量浓度为40 mg/L时,LAX2生长受到明显抑制。矿化产物特征分析结果表明,菌株LAX2可通过碳酸盐矿化作用将Cd矿化为呈小颗粒团聚体的CdCO3晶体。Cd污染稻田土壤经菌株LAX2发酵液、无菌发酵液和菌体细胞修复后,水稻根部Cd含量分别下降了35.3%,19.4%和12.5%,茎叶Cd含量分别降低了26.7%,15.6%和8.4%,水稻籽粒中Cd含量分别降低了28.7%,16.4%和7.5%,土壤有效态Cd含量分别下降了56.9%,34.5%和21.0%。【结论】菌株LAX2可通过碳酸盐矿化作用固结土壤中的有效态Cd,从而减少水稻对Cd的吸收。
关键词:  氧化木糖无色杆菌  生物矿化  土壤有效态Cd  土壤修复
DOI:
分类号:
基金项目:国家自然科学基金项目(51109089);吉林省科技发展计划项目(20160204025SF)
Immobilization of Cd by a carbonate biomineralization bacteria
LI Zhe,WU Di,ZHANG Xiufang,et al
Abstract:
【Objective】This research aimed to investigate the carbonate mineralization of cadmium (Cd) and bioremediation of Cd-polluted paddy soil by strain Achromobacter xylosoxidans LAX2.【Method】LAX2 was inoculated in beef extract peptone liquid medium containing 0-40 mg/L Cd2+,and the tolerance of CX to LAX2 was determined.CdCl2 at concentration of 0.1 mol/L was added to LAX2 fermentation broth for 12 h and the bio-mineralization products were characterized by X-ray diffraction,infrared spectroscopy, scanning electron microscopy and energy spectrum analysis.The remediation of Cd-polluted paddy soil by strain LAX2 was studied using rice pot experiment.【Result】Strain LAX2 had stronger tolerance against Cd2+ and its growth was not affected when Cd2+ concentration was 35 mg/L,while the growth was significantly inhibited when Cd2+ concentration was 40 mg/L.Strain LAX2 could form small particle agglomerated CdCO3 crystals by carbonate mineralization.Cd contaminated rice soil were remediated by the strain LAX2 fermentation broth,sterile fermentation broth and bacterial cells with Cd concentrations in rice roots decreased by 35.3%,19.4% and 12.5%,respectively.The contents of Cd in stem and leaf decreased by 26.7%,15.6% and 8.4%,the contents in rice grains decreased by 28.7%,16.4% and 7.5%,and soil available Cd contents decreased by 56.9%,34.5% and 21.0%,respectively.【Conclusion】Strain LAX2 could immobilize available Cd through carbonate mineralization and reduced Cd uptake by rice.
Key words:  Achromobacter xylosoxidans  biomineralization  available Cd in soil  soil remediation