| 摘要: |
| 【目的】利用大豆基因组Wm82.a2.v1，以拟南芥NUDX基因序列作为参考，通过全基因扫描，在大豆基因组中鉴定大豆NUDX基因家族。【方法】通过比对不同植物NUDXs蛋白序列，分析其保守结构特征，并采用模式匹配的方法分析大豆中具有相同结构的蛋白。利用ProtParam、TargetP1和WoLF-PSORT在线程序，分析候选基因编码蛋白质的亚细胞定位，根据多序列比对结果，利用MEGA5.1进行系统进化分析，最终根据大豆转录组数据对上述挖掘到的基因的表达模式进行分析。【结果】在大豆基因组中共找到69个推定的NUDX基因，分布于20条染色体上，其中56个具有单nudix水解酶结构域。对这69个基因的系统进化分析表明，大部分的GmNUDXs具有2个拷贝，且在大豆基因组上成对出现，这反映出了古老的基因组复制事件。对69个基因的表达分析表明，GmNUDXs在大豆中的表达不具有组织特异性，但表现出了明显的丰度变化：69个基因中：24个基因具有高表达丰度，26个基因具有中等表达丰度，10个基因具有较低表达丰度，9个没有发现表达序列，表明这9个基因可能为假基因。【结论】从大豆基因组数据库中挖掘到69个GmNUDXs，分布于大豆的20条染色体上，在大豆中可能具有多种功能。 |
| 关键词: 大豆 NUDX基因家族 增变基因 系统进化 表达模式 |
| DOI： |
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| 基金项目:河南省科技攻关计划（农业领域）项目（162102110139） |
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| Genome wide analysis of soybean NUDX gene family |
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CHANG Wei,WANG Juan,YU Yang,et al
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| Abstract: |
| 【Objective】In this study,the DNA sequences of soybean genome Wm82.a2.v1 and AtNUDXs were used to identify GmNUDXs in soybean genome.【Method】The NUDXs protein sequences of different plants were compared,the conserved structural characteristics were analyzed and the proteins with same structure were analyzed by matching against the soybean genome database (Wm82.a2.v1).Then,the molecular weight and putative sub-cellular localization of each gene were calculated using online programs ProtParam,TargetP1 and WoLF-PSORT.The phylogenetic tree was generated by MEGA5.1 based on the multiple sequence alignment of the deduced amino acid sequences of all GmNUDXs.At last,the expression patterns of NUDX genes were determined in soybean tissues based on public transcriptome data.【Result】A total of 69 NUDX genes located on all the 20 chromosomes were identified in this study.Fifty six of them encoded proteins with a single nudix hydrolase domain (NHD).The phylogenetic analysis showed that most of the predicted GmNUDXs clustered together in pairs,reflecting the ancient genome duplication event.The expression analysis demonstrated that the expression pattern of most GmNUDXs did not exhibit tissue specificity,but showed a significant abundance variation.Out of the 69 GmNUDXs,24 had high expression abundance,26 were in medium abundance,and 10 were in low abundance.Nine predicted GmNUDXs contained no sequence included in the study.【Conclusion】A total of 69 GmNUDXs on all the 20 chromosomes were identified in this study.Sixty of them were expressed in all tissues,suggesting their versatile role in soybean. |
| Key words: soybean NUDX gene family mutator phylogenetic expression pattern |
