| 摘要: |
| 【目的】研究玉米多药及有毒化合物外排家族（multidrug and toxic compound extrusion,MATE）基因ZmMATE884(GRMZM2G423884)在植物生长发育阶段的功能。【方法】以玉米全基因组DNA为模板克隆玉米MATE蛋白家族基因ZmMATE884，通过蛋白同源比对，预测其可能的跨膜结构域，并通过定量RT-PCR分析该基因的表达模式；构建ZmMATE884融合荧光蛋白瞬时表达载体，转化拟南芥叶肉细胞原生质体，进行亚细胞定位分析；通过农杆菌侵染技术，构建ZmMATE884基因的拟南芥转基因过表达系，观察表型；将ZmMATE884基因的拟南芥转基因过表达系与野生型种子分别种植于含质量分数1%蔗糖和不含糖的1/2 MS固体培养基上，分别黑暗培养6 d和光照培养7 d后，观测统计下胚轴的伸长情况。【结果】ZmMATE884基因在玉米根、上枝叶、下枝叶和胚中有较高表达；ZmMATE884蛋白部分定位于高尔基体/内体上；ZmMATE884基因的拟南芥过表达转基因植株表现为莲座叶发生速率加快且开花提前；在无糖和含质量分数1%蔗糖的1/2 MS固体培养基上黑暗生长6 d及光下生长7 d后，ZmMATE884基因的拟南芥转基因过表达系下胚轴明显短于野生型。【结论】玉米ZmMATE884基因参与了植物生长发育的调控，可加快莲座叶发生，促使植物提前开花，同时还参与下胚轴伸长的负调控。 |
| 关键词: 玉米 MATE蛋白家族 基因表达 亚细胞定位 下胚轴 |
| DOI： |
| 分类号: |
| 基金项目:陕西省自然科学基金项目（2015JM3081） |
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| MATE transporter gene of Zea mays regulates plant development and hypocotyl elongation |
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XUE Shengxiang,JIA Min,YU Fei
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| Abstract: |
| 【Objective】This study explored the function of multidrug and toxic compound extrusion (MATE) family gene ZmMATE884 (GRMZM2G423884) in maize during plant development.【Method】In this paper,the MATE protein family gene ZmMATE884 was cloned from the whole genomic DNA of maize,and the gene expression pattern was analyzed by quantitative RT-PCR.The possible transmembrane domain of ZmMATE884 protein was predicted by protein homology comparison.Transient expression vectors of ZmMATE884 C terminal fused with fluorescent protein were constructed for the transformation of Arabidopsis mesophyll protoplasts to analyze subcellular localization.Then,the ZmMATE884 overexpression transgenic Arabidopsis lines were constructed by Agrobacterium tumefaciens infection technique.Arabidopsis transgenic overexpression lines and wild-type seeds were incubated on 1/2 MS solid medium with and without 1% sucrose for 6 days in the dark or 7 days in the light to observe the hypocotyl elongation.【Result】ZmMATE884 was highly expressed in roots,base leaf,tip leaf and embryos.ZmMATE884 had 12 transmembrane domains,and it was partially localized in golgi and endosomes.The transgenic plants showed faster rosette leaves initiation and earlier flowering.The hypocotyls of the ZmMATE884 gene overexpression transgenic Arabidopsis lines were significantly shorter than that of wild-type.【Conclusion】ZmMATE884 involved in the regulation of plant development and elevated ZmMATE884 expression levels resulted in accelerated plant growth and earlier flowering.In addition,ZmMATE884 was a negative regulator of hypocotyl elongation. |
| Key words: maize MATE family gene expression analysis subcellular localization hypocotyl |
