| 摘要: |
| 【目的】筛选与玉米粒长紧密关联的SNP标记和候选基因，为分子标记辅助选择籽粒较长的玉米材料及克隆相关的功能基因奠定基础。【方法】以80个核心玉米自交系作为关联群体，在2014年和2015年分别将其种植在吉林省长春市和梅河口市，收获后考种。同时采用Illumina Hiseq PE150高通量测序仪对关联群体进行全基因组重测序，将获得的高质量SNP标记用于后续玉米粒长的全基因组关联分析。【结果】不同玉米自交系的籽粒长度遗传变异较大，广义遗传力为83.67%，说明该性状主要受到遗传因素的控制。经过全基因组关联分析，在4个环境下共检测到16个与玉米粒长显著关联(P<10-6)的SNP标记，解释的表型变异率在1.68%～27.61%。其中7个标记位于前人已定位的粒长QTL置信区间内，1个标记在2015年的2个环境中都被检测到。在显著性SNP标记的LD范围内共发掘出GRMZM2G018607、CKMZM2G034882和GRMZM2G32264 3个候选基因，其分别编码ABCF4转运蛋白、WIP2互作蛋白和一个含DUF2346结构域的蛋白质，可能与玉米粒长的发育形成密切相关。【结论】筛选出16个与玉米粒长紧密关联的显著性SNP标记和3个候选基因。 |
| 关键词: 玉米 粒长 全基因组重测序 单核苷酸多态性 全基因组关联分析 候选基因 |
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| 基金项目:吉林省农委资助项目(2012)；农业部“948”项目(2013-Z47) |
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| Genome wide association analysis of maize kernel length |
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DAI Liqiang,WU Lü,DONG Qingsong,et al
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| Abstract: |
| 【Objective】This study screened SNP markers and candidate genes closely linked to kernel length of maize to provide basis for molecular marker assisted selection of long kernel and cloning of related functional genes.【Method】Eighty elite maize inbred lines were selected as the association population,and they were planted in Changchun and Meihekou of Jilin in 2014 and 2015.After harvest,the kernel length was measured,whole-genome resequencing was performed by Illumina Hiseq PE150 high throughput sequencing instrument for association population,and high-quality SNP markers were used for a subsequent genome-wide association study (GWAS).【Result】The genetic variation of kernel length in different maize inbred lines was large,with the broad heritability of 83.67%,indicating that the kernel length was mainly controlled by genetic factors.A total of 16 SNP markers significantly associated with kernel length (P<10-6) were detected by a GWAS in four environments and the phenotypic variation rate was 1.68%-27.61%.Seven SNP markers were located in the range of previously identified QTL,and one SNP marker was detected in both environments in 2015.GRMZM2G018607,CKMZM2G034882 and GRMZM2G32264 genes were identified within the range of linkage disequilibrium for the 16 significant SNP markers.They were predicted to encode ABC transporter F family member 4,WPP domain-interacting protein 2 and DUF2346 domain containing protein,and may be closely linked with kernel length.【Conclusion】Sixteen significant SNP markers and 3 candidate genes closely related to the kernel length of maize were identified. |
| Key words: maize kernel length whole-genome resequencing SNPs genome-wide association study candidate genes |
