| 摘要: |
| 【目的】分析精制橘皮果胶（Purified low molecular citrus pectin，PLMCP）的结构，为靶向性抗癌药物载体的研究提供参考。【方法】以橘皮果胶为原料，制备精制橘皮果胶，采用改良间羟联苯法测定其半乳糖醛酸含量，酸碱滴定法和气相色谱法测定其酯化度，分子排阻色谱法测定其分子质量，单糖乙酰化法测定其单糖含量，分析精制橘皮果胶的主要结构。【结果】精制橘皮果胶半乳糖醛酸含量为95.40%，酯化度为7.99%，分子质量在13.69～24.58 ku，重均分子质量为17.94 ku；单糖只检出半乳糖醛酸，鼠李糖、阿拉伯糖、木糖、葡萄糖和半乳糖均未检出，推测PLMCP为直链结构同型聚半乳糖醛酸。【结论】精制橘皮果胶的结构单一、分布均一、分子质量稳定，可作为新型靶向性抗癌药物的载体。 |
| 关键词: 橘皮果胶 分子质量 半乳糖醛酸 酯化度 |
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| 基金项目:国家“十二五”重大专项（2011ZX09102-001） |
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| Structure analysis on purified low molecular citrus pectin (PLMCP) |
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LIAO Chunmei,TANG Xiaohai,LI Xia,et al
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| Abstract: |
| 【Objective】The structure of purified low molecular citrus pectin (PLMCP) was analyzed to provide basis for the study of targeted anticancer drug carriers.【Method】Purified low molecular citrus pectin was came from the citrus pectin which was purification of raw pectin,the main structure of it was analysis by using the modified hydroxyldiphenyl method for determination the content of galacturonic acid,acid-base titration and gas chromatographythe determination the degree of esterification,used the mass size exclusion chromatography determination of the molecular and acetylation method determination the monosaccharide content.【Result】The content of galacturonic acid in PLMCP was 95.40%,with esterification degree of 7.99%.The molecular weight of PLMCP was 13.69-24.58 ku,and the weight average molecular weight was 17.94 ku.Only glucuronic acid was detected in monosaccharides,while rhamnose,arabiasugar,xylose,glucose,and galactose were not detected.In summary,PLMCP has a straight chain structure with combined galacturonic acids.【Conclusion】PLMCP has a stable molecular weight, uniform distribution and simple structure.Thus,it can be used as a carrier for new anticancer drugs. |
| Key words: citrus pectin molecular weight galacturonic acid degree of esterification |
