| 摘要: |
| 【目的】探讨干旱胁迫下防风保护酶系统与其适应干旱的生理机制。【方法】以2年生防风为材料，于2014-04-28-2015-08-30，采用室外盆栽人工模拟干旱胁迫方法，根据当地(长春市)降水实际，设计3个处理，分别为严重干旱处理（SD，处理期内供水200 mm）、轻度干旱处理（LD，处理期内供水400 mm）、对照处理（CK，处理期内供水600 mm），其灌水量分别相当于全年降水量200,400和600 mm,研究不同干旱胁迫处理对防风保护酶（SOD、POD、CAT）活性和丙二醛（MDA）、脯氨酸及色原酮含量的影响。【结果】干旱胁迫前期，不同处理防风SOD、POD、CAT活性及MDA和脯氨酸含量均呈增加趋势；随着胁迫时间的延长，SOD、POD、CAT活性和脯氨酸含量达到最高值后呈现下降趋势，而MDA含量直到干旱胁迫结束则呈现持续上升趋势。不同干旱胁迫处理防风色原酮含量变化各不相同，随着胁迫时间延长，各处理下防风叶片色原酮含量达到高峰后呈现下降趋势，但LD处理远高于SD和CK处理。随着胁迫程度加重，SOD酶活性与色原酮含量的相关系数不断增大，SD处理下二者呈显著正相关。【结论】在防风栽培过程中，适当控制土壤水分（轻度干旱胁迫）可促进叶片保护酶活性、渗透调节物质含量的提高，有利于防风植株健壮生长并最终提高色原酮的含量。 |
| 关键词: 防风 干旱胁迫 保护酶 脯氨酸 丙二醛 色原酮 |
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| 基金项目:国家自然科学基金项目(31300270);吉林省教育厅“十二五”科学技术研究规划项目(2015205)；吉林省科技发展计划项目(20130522048JH) |
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| Effect of drought stress on physiological characteristics and quality of Saposhnikovia divaricata |
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HAN Zhongming,WANG Yunhe,XU Miaomiao,et al
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| Abstract: |
| 【Objective】The physiological adaptive mechanism and protective enzyme system of Saposhnikovia divaricata under drought stress were investigated.【Method】Biennial Saposhnikovia divaricata was planted by pot cultivation and rainproof was used from 2014-04-28 to 2015-08-30 to study the effects of drought stress on activities of protective enzymes (SOD,POD and CAT) and contents of malondialdehyde (MDA),proline and chromones.The experiments had three treatments including severe drought stress (SD),light drought stress (LD),control treatment (CK) with supply water amounts of 200,400 and 600 mm in the whole year according to actual precipitation in Changchun.【Result】The activities of SOD,POD and CAT and contents of MDA and proline under different drought stresses were increased during early drought stress stage.The activities of SOD,POD and CAT and proline content reached peak values and then decreased with the increase of stress time,but MDA content increased during the whole water drought process.The changes of four chromones were different in different drought stresses,and all chromones decreased after initial increase with the increase of stress time.The chromone contents under LD treatment were higher than those under SD and CK treatments.The correlation coefficients between chromones contents and SOD activities increased with the increase of drought stress,showing extremely significant positive correlation under the SD treatment.【Conclusion】Appropriately controlling soil moisture (slight drought stress) during the cultivation of S.divaricata could increase leaf protective enzyme activities and contents of osmoregulation substances,which are beneficial to S.divaricata growth and increase of chromones contents. |
| Key words: Saposhnikovia divaricata drought stress protective enzyme proline MDA chromone |
