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外源激素对甘蓝DH植株生长的影响
王改改1, 张恩慧1, 李 楠,等1
西北农林科技大学 园艺学院
摘要:
【目的】促进甘蓝胚状体再生DH植株在有限时间内健壮生长,根系粗壮,有利于当年提早移栽,进而植株生长达到通过春化营养体。【方法】以甘蓝小孢子培养获得的胚状体不定芽为试验材料,分别在MS+Suc 30 g/L+Agar 8 g/L培养基上添加不同浓度褪黑素(MT)(0,1,2,5,8和10 μmol/L),在MS +Suc 30 g/L+Agar 8 g/L+5 μmol/L MT培养基上添加不同质量浓度萘乙酸(NAA)(0,0.01,0.05,0.1,0.2和0.5 mg/L),研究再生植株生长势,筛选再生植株快速健壮生长的培养基;再研究分析DH-A、DH-B和DH-C 3种基因型胚状体不定芽在MS+Suc 30 g/L+Agar 8 g/L+5 μmol/L MT+0.1 mg/L NAA培养基上再生植株的生长势,比较MT和NAA对不同基因型DH植株生长的影响。【结果】MS培养基添加MT有利再生植株生长,与对照相比,培养基添加5 μmol/L MT处理再生植株净生长高度、茎粗、最大叶宽和叶片数分别增加1.33 cm,0.17 mm,0.67 cm和2.60片,根系变长、侧根增多,翌年抽苔率提高20.0%;MS培养基添加5 μmol/L MT 和0.1 mg/L NAA后再生植株健壮生长,与对照相比,再生植株净生长高度、茎粗、最大叶宽和叶片数分别增加1.90 cm,0.52 mm,0.88 cm和3.00片,根系生长旺盛,翌年抽苔率提高26.6%;5 μmol/L MT和0.1 mg/L NAA对甘蓝DH植株的促进作用在3种不同基因型间无显著性差异,且DH-A、DH-B、DH-C翌年抽苔率分别较对照提高33.4%,20.0%和20.0%。【结论】在MS培养基中添加5 μmol/L MT和0.1 mg/L NAA,甘蓝胚状体DH植株生长势最好,当年炼苗移栽,冬前植株生长可达到一定的营养体。
关键词:  甘蓝  褪黑素  胚状体  不定芽  DH植株  植株生长
DOI:
分类号:
基金项目:国家重点研发计划项目(2016YFD0101702);陕西省农业科技创新与攻关项目(20016NY-046)
Effect of melatonin on growth of cabbage DH plant
WANG Gaigai,ZHANG Enhui,LI Nan,et al
Abstract:
【Objective】This study aimed to improve the growth of cabbage regeneration DH plant induced by embryoid in limited period and achieve vegetation by vernalization.【Method】The adventitious buds from cabbage microspore culture were planted in MS+Suc 30 g/L+Agar 8 g/L medium with different concentrations of melatonin (MT) (0,1,2,5,8,and 10 μmol/L).In MS+Suc 30 g/L+Agar 8 g/L+5 μmol/L MT medium, different concentrations of NAA (0,0.01,0.05,0.1,0.2 and 0.5 mg/L) were also supplemented. The effects of MT and NAA on growth of different genotypes DH plants were compared and the growth potential of adventitious buds regenerated plantlets of DH-A,DH-B,and DH-C in MS+Suc 30 g/L+Agar 8 g/L+5 μmol/L MT+0.1 mg/L NAA medium were analyzed.【Result】The MS medium supplemented with MT was advantaged to growth and development of regeneration plants,and the net growth height,stem diameter,maximum width,number of leaf and rate of bolting in the following year were increased by 1.33 cm,0.17 mm,0.67 cm,2.60 and 20.0%,and longer root and more lateral roots were obtained when compared to the control.The regenerated plants grew vigorously when the medium MT and NAA were added,and the net growth of height,stem diameter,maximum width,number of leaf and rate of bolting in the following year were increased by 1.90 cm,0.52 mm,0.88 cm,3.00 and 26.6%,respectively.There was no significant difference in the promoting effect,and the rates of bolting of DH-A,DH-B,DH-C in the following year were increased by 33.4%,20.0% and 20.0% with 5 μmol/L MT and 0.1 mg/L NAA on the three genotype cabbage DH plants,respectively. 【Conclusion】The growth vigor of cabbage regeneration DH plant in MS medium supplemented with 5 μmol/L MT and 0.1 mg/L NAA was the best to achieve definite vegetation before winter.
Key words:  cabbage  melatonin  embryoid  adventitious buds  DH plant  plant growth