| 摘要: |
| 【目的】研究霜霉威在花椰菜上的消解动态及最终残留量，评价其在花椰菜上使用的安全性，为在花椰菜上的科学合理使用及霜霉威限量标准研究提供依据。【方法】建立测定花椰菜中霜霉威的残留量色谱 质谱法；按照农药残留试验准则，于2013和2014年在河北保定、陕西西安、四川广元进行霜霉威在花椰菜上残留的田间试验，采集样品并经乙酸乙酯匀浆提取和液-液萃取净化，减压浓缩定容后，采用液相色谱-质谱法进行霜霉威残留量检测。【结果】霜霉威在花椰菜植株及可食用部分中的添加回收率分别为85.9%~104.5%， 87.5%~101.5%，其在花椰菜植株及可食用部分中的最低检出含量均为0.05 mg/kg，最低检出量均为0.25 ng。2013和2014年，霜霉威在花椰菜植株中的原始残留量分别为4.68和1.36 mg/kg，半衰期分别为6.0和5.3 d，属于易降解农药。2013和2014年最终残留试验结果表明，于最后一次施药后的5，7和10 d，霜霉威在花椰菜可食用部分中的最终残留量最大值分别为0.94和0.76，0.62和0.55，0.52和0.36 mg/kg。【结论】成功建立了检测农产品中残留的霜霉威的液相色谱-质谱法；霜霉威在花椰菜上的最大残留量均低于欧盟的标准，属于易降解农药，在花椰菜上使用安全。 |
| 关键词: 霜霉威 花椰菜 消解动态 最终残留 |
| DOI： |
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| 基金项目:农业部蔬菜联合试验项目（071-2013蔬菜联合试验-1405） |
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| Degradation dynamics and final residues of propamocarb in cauliflower |
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YU Fuli,WANG Yuping,LEI qi,et al
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| Abstract: |
| 【Objective】The degradation and final residues of propamocarb in cauliflower were investigated to evaluate its application safety.【Method】The method of LC-MS/MS was established for determination of propamocarb residues in cauliflower.Field experiments at Baoding of Hebei,Xi’an of Shaanxi,and Guangyuan of Sichuan were conducted in two years,and the plant samples were collected at different dates.Propamocarb was extracted with ethylacetate and cleaned by liquid liquid extraction.After the solvent was evaporated in vacuum,the residue pesticide was dissolved and detected by LC-MS/MS.【Result】The recoveries of propamocarb were 85.9%-104.5% and 87.5%-101.5% in plants and cauliflowers,respectively.LOQ and LOD of propamocarb were 0.05 mg/kg and 0.25 ng in leaves and cauliflowers,respectively.Propamocarb can be rapidly degraded in cauliflower,and the original deposit amounts were 4.68 and 1.36 mg/kg in 2013 and 2014.The half life (t1/2) in leaves were 6.0 and 5.3 d at 2013 and 2014,respectively.Final residues of propamocarb in cauliflower 5,7 and 10 d after the last spraying were 0.94 and 0.76，0.62 and 0.55 mg/kg，0.52 and 0.36 mg/kg,respectively.【Conclusion】The LC-MS/MS method was successful established for determination of propamocarb residues in agricultural products. The propamocarb residual in cauliflower was below the EU standards,and it is degradable and safe to be used for cauliflowers. |
| Key words: propamocarb cauliflower degradation dynamics final residue |
