| 摘要: |
| 【目的】利用AFLP标记和ITS序列分析方法，对11份荆芥（Schizonepeta tenuifolia Briq.）种质进行分析鉴定，为荆芥种质资源鉴定和利用提供理论依据。【方法】以11份荆芥幼苗为材料，提取基因组DNA，利用6种引物组合对其进行AFLP标记分析。利用ITS引物对荆芥样品进行PCR扩增、测序，利用DNAMAN软件分析11份种质的ITS序列，对其进行鉴定。【结果】荆芥AFLP标记多态性不高，选用的6个引物组合扩增出条带241条，多态性条带154条，多态性比率为63.90%；对供试材料AFLP数据结果进行聚类，亲缘关系较近或引种频繁地区荆芥种质资源聚类在一起。ITS序列分析结果表明，供试的11份荆芥材料的ITS序列完全相同，表明荆芥种质间ITS序列保守性较强。【结论】同时利用AFLP标记和ITS序列分析对荆芥种质资源进行鉴定，可以有效提高检测的准确性。 |
| 关键词: 荆芥 AFLP ITS 种质资源 |
| DOI： |
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| 基金项目:河北省教育厅项目（ZC2016004）；唐山市科技局项目（15130266a） |
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| Diversity of Schizonepeta tenuifolia Briq.based on AFLP and ITS sequence analysis |
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MA Yanzhi
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| Abstract: |
| 【Objective】The aim of this study was to identify and analyze 11 Schizonepeta tenuifolia Briq.germplasms using AFLP and ITS sequence analysis,which would provide theoretical basis for resource identification and utilization.【Method】Eleven Schizonepeta tenuifolia Briq.cultivars were selected and DNA was extracted from seedlings.Six primer sets were used to conduct AFLP PCR amplification.ITS sequences of the cultivars were then obtained,and the DNAMAN software was employed to align the ITS sequences.【Result】A total of 241 DNA bands were amplified from the 6 primer combinations including 154 polymorphic bands with the polymorphic ratio of 63.90%,which was not high.Based on AFLP data,clustering analyses on the 11 materials were carried out.Germplasm resources were clustered together based on close relationship or introduction frequent area.ITS sequence analysis showed that there was no difference among the 11 Schizonepeta tenuifolia Briq.cultivars,indicating that the ITS sequences of Schizonepeta tenuifolia cultivars were highly conservative.【Conclusion】Simultaneous application of AFLP and ITS sequence analysis remarkably improved the accuracy of Schizonepeta tenuifolia Briq.identification. |
| Key words: Schizonepeta tenuifolia Briq. AFLP ITS genetic diversity |
