| 摘要: |
| 【目的】研究日粮蛋白水平和采精频率对陕北白绒山羊种公羊血清生化指标和生殖激素的影响，进一步研究种公羊蛋白需要并确定适宜的采精频率，进而为陕北白绒山羊种公羊饲养标准制定提供科学依据。【方法】试验选取12只健康无病陕北白绒山羊种公羊，采用随机区组设计分为4组，每组3只，分别饲喂4种蛋白水平（5.10%，7.11%，8.07%，9.35%，质量分数）日粮，并在配种正试期分别采用5 d 2次和5 d 4次的采精频率。饲养试验结束时，进行颈静脉采血，测定血清生化指标和生殖激素含量，分析日粮蛋白水平和采精频率对种公羊血清生化指标及生殖激素的影响。【结果】随着日粮蛋白水平增加，血清尿素氮（BUN）浓度显著升高（P<0.05），日粮蛋白水平对血糖（Glu）、总蛋白（TP）、白蛋白（ALB）、球蛋白（GLB）、白球比（A/G）、甘油三酯（TC）、总胆固醇（TG）及生殖激素含量影响总体不显著（P>0.05）。采精频率对种公羊睾酮质量浓度有显著影响(P<0.05)，但日粮蛋白水平与采精频率之间不存在显著交互作用。【结论】陕北白绒山羊种公羊在配种期日粮蛋白水平为8.07%，采精频率为5 d 2次，种公羊可保持较高的睾酮水平。 |
| 关键词: 日粮蛋白水平 陕北白绒山羊种公羊 采精频率 血清生化指标 生殖激素 |
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| 基金项目:国家绒毛用羊产业技术体系项目（CARS-40-13）；陕西省农业科技攻关项目（2014K01-17-04）；国家公益性行业（农业）科研专项（201303059） |
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| Effects of dietary protein level and semen collection frequency on serum biochemical parameters and reproductive hormones of Cashmere goat bucks |
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WANG Xingtao,LEI Yaogeng,LI Bibo,et al
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| Abstract: |
| 【Objective】The research investigated the effects of dietary protein level and semen collection frequency on serum biochemical parameters and reproductive hormones of Northern Shaanxi White Cashmere goat bucks and determined protein requirements and appropriate semen collection frequency of bucks to provide scientific basis for feeding standards.【Method】Twelve healthy bucks were selected and allocated to 4 groups with 3 per group by randomized block design.Bucks in different groups were fed with different protein levels of 5.10%,7.11%,8.07% and 9.35%,respectively.The semen collection frequency was set to two times of every five days or four times of every five days during breeding season.At the end of trial,blood was collected from jugular venous to determine the effect of dietary protein level and semen collection frequency on serum biochemical parameters and reproductive hormones.【Result】The concentrations of blood urine nitrogen were increased with the increase of dietary protein level during all periods significantly (P<0.05),while dietary protein levels had no influence on the concentrations of Glu,BUN,TP,ALB,GLB,A/B,TC,TG,and reproductive hormone (P>0.05).Semen collection frequency affected the concentrations of testosterone significantly (P<0.05),but there was no interaction between dietary protein levels and semen collection frequency.【Conclusion】Goat bucks could keep concentrations of serum testosterone when the dietary protein content was 8.07% and the semen collection frequency was two times of every five days during breeding season. |
| Key words: protein levels Northern Shaanxi White Cashmere goat buck semen collection frequency biochemical parameters reproductive hormones |
