| 摘要: |
| 【目的】探讨蜡蚧轮枝菌生物学特性与菌株对榕母管蓟马毒力的相关性，为榕母管蓟马优质高效生防菌株的初步筛选提供参考。【方法】室内观察蜡蚧轮枝菌V07、V16063、V3450和Vp28 4株菌株的生物学特性，测定其对榕母管蓟马成虫的毒力，通过逐步回归分析探讨两者间的相关性。【结果】4株蜡蚧轮枝菌菌株的生物学特性存在异同，产孢量和孢子萌发率均很高，但V3450菌株生长速度最快，孢子GT50最短；V16063和Vp28菌株生长速度较快，孢子GT50较短；V07菌株生长速度最慢，孢子GT50最长。榕母管蓟马成虫受供试菌株侵染后的死亡趋势均随菌剂浓度升高和侵染时间延长而上升，但不同菌剂中的变幅则明显不同，侵染致死效果依次为V3450＞V16063＞Vp28＞V07，LT50依次为V3450＜V16063＜Vp28＜V07。逐步回归分析表明，菌株生长速度和孢子GT50对累计校正死亡率和LT50的作用均达显著水平。【结论】在供试菌株产孢量和孢子萌发率均很高时，菌株生长速度和孢子GT50可作为评判蜡蚧轮枝菌对榕母管蓟马毒力大小的初筛指标。 |
| 关键词: 蜡蚧轮枝菌 生物学特性 榕母管蓟马 成虫毒力 |
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| 基金项目:福建省科技重大专项（2013NZ0002-4）；福建省属公益优势领域重点项目（2014R1024-1）；福建省自然科学基金项目（2014J01110）；福建省科技型中小企业技术创新资金项目（2015C0027） |
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| Correlation between biological characteristics of Lecanicillium lecanii and the virulence against Gynaikothrips ficorum |
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HUANG Peng,YU Deyi,YAO Jin’ai,et al
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| Abstract: |
| 【Objective】This study explored the correlation between biological characteristics of Lecanicillium lecanii and the virulence against Gynaikothrips ficorum to provide reference for preliminary screening of high quality and efficient entomogenous fungi against G.ficorum.【Method】The biological characteristics of four L.lecanii strains (V07,V16063,V3450 and Vp28) were observed and the virulence against Gynaikothrips ficorum was determined.Then,the correlation was analyzed by stepwise regression analysis.【Result】There were similarities and differences among biological characteristics of four L.lecanii strains.They all had high sporulation quantity and conidia germination rate.Strain V3450 had the fastest growth rate and the shortest conidia GT50.Strains V07 had the slowest growth rate and the longest conidia GT50.Growth speed and conidia GT50 of strains V16063 and Vp28 were in middle.The death trend of G.ficorum adults increased with the increase of concentration and infection time.The sequence of infection lethal effect was V3450＞V16063＞Vp28＞V07 while the sequence of LT50 was V3450＜V16063＜Vp28＜V07.Stepwise regression analysis showed that growth speed and conidia GT50 significantly affected corrected accumulative mortality and LT50.【Conclusion】Strain growth rate and conidia GT50 could be used as preliminary screening indices for evaluating the virulence of L.lecanii against G.ficorum when strain sporulation quantity and conidia germination rate were high. |
| Key words: Lecanicillium lecanii biological characteristics Gynaikothrips ficorum adult virulence |
