| 摘要: |
| 【目的】探讨Nesfatin-1在大鼠生长轴（下丘脑、腺垂体、肝脏、胰腺、肌肉）中的定位及其mRNA的表达情况。【方法】对雌性大鼠进行深度麻醉灌注后，采集下丘脑、腺垂体、肝脏、胰腺、腓肠肌肌肉组织，放入4%多聚甲醛溶液中固定，采用免疫组化PV-9000二步法、DAB显色技术检测Nesfatin-1在生长轴上的分布，另取雌性大鼠断头处死，取相同组织用荧光定量PCR方法检测其中Nesfatin-1 mRNA的表达情况。【结果】Nesfatin-1主要分布于下丘脑的室旁核、背内侧核、室周核、外侧区、弓状核、视上核、腺垂体、胰腺的胰岛和腺泡及肝脏与肌肉中。平均光密度分析显示，Nesfatin-1在室周核和胰岛的表达量显著地高于其他组织（P<0.05），在胰腺腺泡、肝脏及肌肉中的表达量则显著地低于其他组织（P<0.05），在下丘脑背内侧核、外侧区和室旁核中的表达量显著高于视上核、弓状核和腺垂体（P<0.05）。Nesfatin-1 mRNA在胰腺中表达量最高，腺垂体次之，下丘脑和肝脏较低，在胰腺和腺垂体的表达量显著高于下丘脑和肝脏，但下丘脑与肝脏差异不显著（P>0.05）；在腓肠肌中未检测出Nesfatin-1 mRNA的表达。【结论】Nesfatin-1及其mRNA在生长轴中的广泛表达，表明Nesfatin 1可能对大鼠生长发育具有调控作用。 |
| 关键词: Nesfatin-1 大鼠 生长轴 表达与调控 |
| DOI： |
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| 基金项目:高等学校博士学科点专项科研基金博导类资助项目(20123418110004) |
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| Expression of Nesfatin-1 and its mRNA in growth axis of rats |
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LI Ran,WANG Yaoyao,LI Yunsheng,et al
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| Abstract: |
| 【Objective】In the paper,expression of Nesfatin-1 mRNA in growth axis of rats including hypothalamus,pituitary,liver,pancreas,and muscle was explored.【Method】After deep anesthesia perfusion,hypothalamus,adenohypophysis,liver,pancreas,and gastrocnemius muscle of female rats were collected and fixed in 4% paraformaldehyde solution.Then,immuno-histochemical PV-9000 2-step method and DAB staining technique were used to detect the distribution of Nesfatin-1.Other female rats were beheaded and same tissues were collected and the expression was measured using fluorescence quantitative polymerase chain reaction (PCR).【Result】Nesfatin-1 mainly distributed in the hypothalamic paraventricular nucleus (PVN),dorsomedial nucleus(DMH),periventricular nucleus(PeN),lateral hypothalamic area(LHA),arcuate nucleus(ARC),supraoptic nucleus(SON),pituitary,pancreas islet and acinar cells,liver,and muscle.Average optical density of Nesfatin-1 in PeN and pancreatic islet was significantly higher (P<0.05) than in other tissues while that in pancreatic acini,liver and muscle was significantly lower (P<0.05).The expression in hypothalamic DMH,LHA and PVN was significantly different from in SON,ARC and anterior pituitary.The expression level of Nesfatin-1 mRNA in pancreas was the highest followed by pituitary,while the expression in liver and hypothalamus was the lowest.No expression of Nesfatin-1 mRNA was observed in muscle.【Conclusion】Nesfatin-1 and its mRNA widely distribute in the growth axis,indicating that Nesfatin-1 may participate in regulation of growth and development in rats. |
| Key words: Nesfatin-1 rat growth axis expression and regulation |
