| 摘要: |
| 【目的】筛选皖南花猪和大约克猪背最长肌组织差异表达基因。【方法】采集皖南花猪和大约克猪背最长肌（各3头），测定其肉质性状指标，并提取其RNA，采用高通量转录组测序(RNA-seq)技术进行测序，对所获序列进行GO和Pathway显著性富集分析。【结果】测序后皖南花猪3个样本得到的总序列数分别为65 584 126，64 327 738和59 244 502，其中有效序列达到94.4%，94.3%和94.2%；大约克猪3个样本得到的总序列数分别为57 969 134，68 254 168和72 298 142，其中有效序列达到93.8%，93.7%和93.8%。测序饱和度良好，证明测序数据真实可靠。差异表达分析结果显示，共筛选到347个差异表达基因，其中包含94个上调基因，253个下调基因。GO 功能显著性富集和Pathway显著性富集分析发现，差异表达基因富集在与糖酵解代谢和肌肉发育相关的生物学过程中以及与脂肪酸代谢和生长性状、胴体性状相关的信号通路上。【结论】获得了猪背最长肌组织基因表达谱，筛选出了一些与肉质性状和生长性状有关的候选基因。 |
| 关键词: RNA-seq 皖南花猪 大约克猪 差异表达基因 背最长肌 |
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| 基金项目:安徽省生猪产业技术体系项目（AHCYTX-06-10）；安徽省生猪现代农业项目（13000101）；安徽省农业科学院科技创新团队建设项目(13C0405) |
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| Screening and annotation of differentially expressed genes in longissimus muscle of different pig breeds based on RNA-seq technology |
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LI Xiaojin,QIAN Kun,LIU Linqing,et al
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| Abstract: |
| 【Objective】This study aimed to find differentially expressed genes in longissimus muscle of Wannanhua pig and Yorkshire pig.【Method】This experiment selected 3 Wannanhua pigs and 3 Yorkshire pigs to obtaine longissimus muscles.Meat quality traits were measured,RNA was extracted,high-throughput transcriptome sequencing (RNA-seq) technology was used to sequence,and GO function annotation and pathway on different genes were analyzed.【Result】Total numbers of sequencing reads of the three Wannanhua pig samples were 65 584 126,64 327 738 and 59 244 502,respectively.The percentages of clean reads reached 94.4%,94.3% and 94.2%.Total numbers of sequencing reads of the three Yorkshire pig samples were 57 969 134,68 254 168 and 72 298 142,respectively.The percentages of clean reads reached 93.8%,93.7% and 93.8%.The high saturation degree of sequencing demonstrated that the data were realistic and reliable.Differential expression analyses showed that there were 347 differentially expressed genes,in which 94 were up-regulated and 253 genes were down-regulated.Gene Ontology functional enrichment and pathway enrichment analyses revealed that the differentially expressed genes were primarily associated with glycolytic metabolism,biological processes of muscle development and signal pathways related to fatty acid metabolism,growth and carcass traits.【Conclusion】This study obtained the gene expression profile of porcine longissimus dorsi muscle tissue and identified novel candidate genes for porcine meat quality and growth traits. |
| Key words: RNA-seq Wannanhua pig Yorkshire pig differentially expressed genes longissimus muscle |
