| 摘要: |
| 【目的】分析盐碱浓度、胁迫时间对不同水稻品种各龄苗有机渗透调节物质的影响及不同品种对盐碱胁迫响应的差异，为盐碱地改良水稻品种的选择及其敏感期防护提供理论依据。【方法】选择耐盐碱水稻品种长白9号和普通栽培品种吉粳78为材料，研究3个盐碱浓度(10，40，80 mmol/L)、4个处理时间(24，48，72，84 h)对3种苗龄(30，45，70 d)水稻叶片脯氨酸、甜菜碱、肌醇含量及Δ1-吡咯琳-5-羧酸合成酶(P5CS)、甜菜碱醛脱氢酶（BADH1、BADH2）、肌醇单磷酸酶(IMP)基因表达量的影响，以正常水培的水稻为对照。【结果】正常水培条件下长白9号叶片脯氨酸、甜菜碱含量高于吉粳78。盐碱胁迫条件下，长白9号和吉粳78叶片的脯氨酸、甜菜碱和肌醇含量均上升，P5CS、BADH1、BADH2、IMP基因表达量也高于对照。40 mmol/L NaCO3-NaHCO3处理较10和80 mmol/L处理更有利于植株积累渗透调节物质及上调相关基因的表达。长白9号渗透调节物质含量及其基因表达量的变化远大于吉粳78。盐碱胁迫24和48 h能使长白9号及时做出响应，并随着胁迫时间的延长甜菜碱、肌醇含量及BADH1、BADH2、IMP表达量持续升高，而吉粳78对盐碱胁迫响应较晚。2种水稻30，45 d龄苗渗透调节物质含量的变化及相关基因表达量的变化均比70 d明显。【结论】长白9号耐盐碱的特性与其植株体内渗透调节物质丰富且对盐碱胁迫响应快的生理现象有关。2种水稻对盐碱胁迫具有一致的生理响应敏感阈值，在耐受条件下，盐碱地种植水稻更适宜大苗移栽。 |
| 关键词: 水稻 盐碱胁迫 渗透调节物质 脯氨酸 甜菜碱 肌醇 |
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| 基金项目:吉林省自然科学基金项目（201215183）；吉林省教育厅重点项目（2012044）；吉林省博士后科研启动基金项目 |
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| Changes in osmolytes and related genes of different rice varieties under saline-alkali stress |
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ZHANG Tingting,YANG Meiying,WANG Chunhong,et al
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| Abstract: |
| 【Objective】This study analyzed the changes in osmolytes and genes expressions of 2 rice varieties under different saline-alkali concentrations and stress times to provide basis for species selection and plant protection.【Method】Rice varieties Changbai 9 (saline-alkali-tolerant) and Jijing 78 (normal) were selected to study the changes in contents of proline,betaine and inositol and expressions of P5CS,BADH1,BADH2,and IMP in leaves of seedlings at 3 different ages (30,45,70 d) under 3 NaCO3-NaHCO3 concentrations (10,40,80 mmol/L) and 4 saline alkali stress times (24,48,72,84 h).【Result】The contents of proline and betaine in leaves of Changbai 9 were higher than that of Jijing 78 under normal hydroponic condition.With saline alkali stress,the contents of proline,betaine and inositol in leaves of both Changbai 9 and Jijing 78 increased and the expressions of P5CS,BADH1,BADH2,and IMP were also higher than CK.The treatment with 40 mmol/L NaCO3-NaHCO3 was more conducive to accumulate osmolytes and regulate gene expression than treatments with 10 and 80 mmol/L NaCO3-NaHCO3.However,the changes in osmolytes contents and genes expressions in Changbai 9 leaves were more significant than that of Jijing 78.Changbai 9 responded in time after 24 and 48 hours stress and the contents of betaine and inositol as well as expression levels of BADH1,BADH2,and IMP increased continuously with the extension of stress time.Jijing 78 behaved differently and slowly.The seedlings at the ages of 30 and 45 days had more significant responses than that at the age of 70 days.【Conclusion】Changbai 9 could tolerate high saline alkali stress because it is rich in osmolytes and responded quickly to stress.The sensitive thresholds of both rice varieties were consistent,and saline-alkali-tolerant variety was more suitable for large seedling transplanting within thresholds. |
| Key words: rice saline alkali stress osmolytes proline betaine inositol |
