| 摘要: |
| 【目的】对甲酚纳米乳消毒剂进行质量评价和消毒功效研究。【方法】分别利用肉眼观察和离心法检测甲酚纳米乳消毒剂的外观性状和物理稳定性，染色法和稀释法鉴别其结构类型，透射电镜观察其微观形态，激光粒度分析仪测定其粒径大小，加速试验考察其稳定性，最后通过载体定量杀菌试验和物体表面现场消毒试验研究甲酚纳米乳消毒剂的消毒功效。【结果】甲酚纳米乳消毒剂外观澄明、均一，离心不分层，为水包油型纳米乳，乳滴呈规则的球形，平均粒径为（34.89±3.56） nm，粒径呈正态分布；在37 ℃贮存90 d，主药甲酚的平均含量下降了9.62%，稳定性良好。体积分数0.5%和1.0%的甲酚纳米乳消毒剂分别作用3和1 min以上时，对大肠杆菌和金黄色葡萄球菌的平均杀灭率均大于99.90%；体积分数1%和高于3%的甲酚纳米乳消毒剂分别作用5和3 min以上时，对自然菌的杀灭率均大于90%；甲酚纳米乳消毒剂的消毒效果与来苏儿无显著差异（P>0.05）。【结论】甲酚纳米乳消毒剂水溶性好，刺激性气味小，稳定性好，消毒功效强，值得在临床中推广应用。 |
| 关键词: 甲酚 纳米乳 消毒剂 中和剂 |
| DOI： |
| 分类号: |
| 基金项目:新乡市科技发展计划项目（13NY29）；国家级大学生创新创业训练计划项目（201210467037） |
|
| Quality evaluation and disinfection efficacy of cresol nanoemulsion disinfectant |
|
YANG Xue-feng,HAN Qing-gong,ZHENG Ai-wu,et al
|
| Abstract: |
| 【Objective】The aim of this study was to evaluate the quality and disinfection efficacy of novel cresol nanoemulsion disinfectant.【Method】The external properties,physical stability,structure type,micromorphology,particle size,and stability of cresol nanoemulsion disinfectant were determined by macroscopic observation,centrifuge method,staining/dilution method,transmission electron microscope,laser particle size analyzer,and acceleration test,respectively.The carrier quantify disinfect experiment and body surface disinfection test were also conducted to evaluate the disinfection efficacy of the disinfectant.【Result】Cresol nanoemulsion disinfectant was clear,uniform,and not creamed after centrifugalization.The emulsion belonged to oil in water type and the droplet was regular sphere.The mean droplet size was (34.89±3.56) nm with normal distribution.The average content of cresol reduced by 9.62% after storing for 90 days at 37 ℃,indicating that it was stable.The average killing rates of cresol nanoemulsion disinfectants with concentrations of 0.5% and 1.0% (V/V) against E.coli and S.aureus were all higher than 99.90% for more than 3 and 1 min,respectively.The average killing rates against natural bacteria were all higher than 90% when disinfectants with concentrations of 1% and >3% (V/V) were used for over 5 and 3 min,respectively.The difference in disinfection effects of cresol nanoemulsion disinfectant and Lysol was not significant (P>0.05).【Conclusion】Cresol nanoemulsion disinfectant had good solubility,small pungent taste,good stability and strong disinfection efficacy.Thus,it can be generalized and applied in clinic. |
| Key words: cresol nanoemulsion disinfectant neutralizer |
