| 摘要: |
| 【目的】探讨钙处理对低温胁迫下枇杷叶片Ca2+-ATPase活性和膜脂过氧化的调节机制，为枇杷抗寒性研究提供依据。【方法】采用外源钙(5 mmol/L CaCl2)和钙调素拮抗剂三氟拉嗪(Trifluoperazine,TFP，100 mmol/L)处理Hoagland营养液砂培法培养的2年生“早钟6号”枇杷(Eriobotrya japonica Lindl.cv.Zaozhong No.6)容器幼苗，设钙预处理、TFP预处理和先TFP后钙处理，以未做预处理为对照，于－6 ℃人工气候室进行低温胁迫处理，研究钙处理对低温胁迫下枇杷成活率、CaM含量以及Ca2+ ATPase、CAT、SOD活性和H2O2、MDA含量的影响。【结果】与对照相比，钙处理显著提高了低温胁迫后枇杷幼苗的成活率、叶片细胞CaM含量以及质膜、内质网膜、液泡膜和线粒体膜Ca2+-ATPase活性，并激活了CAT和SOD活性，降低了细胞H2O2和MDA含量；而钙调素拮抗剂TFP对钙处理所诱导的上述生理效应均有抑制作用，削弱了幼苗对低温胁迫的抗氧化能力，加剧了低温对幼苗的伤害。【结论】钙处理提高了枇杷幼苗的抗冻能力,Ca2+ CaM信使系统可能参与了低温胁迫下枇杷抗冻性的诱导和调控。 |
| 关键词: 枇杷 低温胁迫 钙调蛋白 Ca2+-ATPase 膜脂过氧化 |
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| 基金项目:福建省科技厅重点项目(2011N0028);福建省高校服务海西建设重点项目(2008HX02) |
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| Effects of calcium on Ca2+-ATPase activity and lipid peroxidation level of loquat seedling under low temperature stress |
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WU Jin-cheng,CHEN Yu,WU Bi-sha,et al
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| Abstract: |
| 【Objective】The regulation mechanism of calcium on Ca2+-ATPase activity and lipid peroxidation of loquat under low temperature stress was studied to provide basis for cold resistance of loquat.【Method】Tow-year-old ‘Zaozhong No.6’ loquat (Eriobotrya japonica Lindl.) seedlings planted in containers with sand culture method and Hoagland nutrition solution were treated with 5 mmol/L CaCl2 and 100 mmol/L Trifluoperazine (TFP).The seedlings with pretreatment with calcium,pretreatment with TFP,pretreatment with TFP after calcium and non pretreatment (CK) were subjected to low temperature (-6 ℃) stress in a phytotron for 6 h to investigate the effects on survival rate,activities of Ca2+-ATPase,catalase (CAT) and superoxide dismutase (SOD),as well as contents of CaM,H2O2 and MDA.【Result】The survival rate of seedlings,CaM content,and Ca2+-ATPase activities of plasma membrane,endoplasmic membrane,tonoplast membrane and mitochondrial membrane in leaf cells were markedly enhanced by Ca2+ treatment.The treatment also stimulated the activities of CAT and SOD while reduced the contents of H2O2 and MDA.The above physiological effects were all inhibited by TFP,which weakened the antioxidant capacity of loquat seedlings under low temperature stress and aggravated the damage.【Conclusion】Ca2+ treatment enhanced the cold resistance of loquat seedlings.Signal system of Ca2+·CaM may induce and regulate the cold resistance of loquat under low temperature stress. |
| Key words: loquat (Eriobotrya japonica Lindl.) low temperature stress calmodulin (CaM) Ca2+-ATPase lipid peroxidation |
