| 摘要: |
| 【目的】研究嫁接对铜胁迫下甜瓜幼苗生长发育与内源激素水平的影响。【方法】试验以南瓜‘京欣砧3号’为砧木，薄皮甜瓜‘IVF09’为接穗，自根苗为对照，测定不同Cu2+浓度（0，400，800 μmol/L）胁迫下甜瓜自根苗和嫁接苗生长指标、激素水平和气孔导度的变化。【结果】在铜胁迫条件下，甜瓜幼苗生长受到抑制、叶片内源激素水平改变，但在同一浓度Cu2+胁迫条件下嫁接苗的生物量大于自根苗。嫁接苗叶片中IAA含量显著高于自根苗，ABA、GA3、ZR含量均显著低于自根苗，其中在800 μmol/L Cu2+胁迫下嫁接苗叶片IAA、ZR含量与自根苗的差异最大，为自根苗的1.30和0.77倍，而在400 μmol/L Cu2+胁迫下，嫁接苗的ABA、GA3含量与自根苗的差异最大，为自根苗的0.68和0.92倍。嫁接苗叶片的ABA/IAA、ABA/GA3、ABA/ZR值均小于自根苗，且嫁接苗叶片中ABA/IAA和ABA/GA3值变化幅度小于自根苗。铜胁迫下，甜瓜嫁接苗气孔导度（Gs）与ABA/ZR值呈极显著负相关，嫁接苗气孔导度的调节受到了ABA和ABA/ZR值的调控，从而维持了较高的气孔导度，增强了甜瓜对铜胁迫的耐性。【结论】嫁接可通过改变内源激素含量而增强甜瓜幼苗对铜胁迫的耐受性，并且有效维持植物内源激素的平衡，促进甜瓜幼苗正常生长。 |
| 关键词: 甜瓜 嫁接 铜胁迫 内源激素 |
| DOI： |
| 分类号: |
| 基金项目:四川省教育厅项目(10ZB044） |
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| Effects of grafting on growth and endogenous hormones of melon (Cucumis melo L.) seedlings under copper stress |
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TAN Ming-ming,TANG Jian,HE Zhong-qun,et al
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| Abstract: |
| 【Objective】The effects of grafting on endogenous hormones of melon seedlings under copper stress were investigated in this study.【Method】Using pumpkin ‘Jingxinzhen No.3’ as stock,melon ‘IVF09’ as cion,and un-grafted seedlings as control,biomass,hormone level and stomatal conductance of melon were measured under different Cu2+ concentrations （0,400,and 800 μmol/L）.【Result】Under copper stress,the growth of melon seedlings was inhibited,and endogenous hormone levels in leaves were changed.Under same stress,the biomass of grafted seedlings was greater than that of un-grafted seedlings.IAA content in grafted seedlings was significantly higher than that of ungrafted one,but ABA,GA3,and ZR contents in leaves of grafted seedlings were significantly lower than that of un-grafted ones.Differences in IAA and ZR contents in leaves of grafted and ungrafted seedlings reached the peak under the 800 μmol/L Cu2+ stress,which were 1.30 and 0.77 times of that of ungrafted seedlings.Differences in ABA and GA3 contents between grafted and ungrafted seedlings reached the peak under the 400 μmol/L Cu2+ stress,which were 0.68 and 0.92 times of that of ungrafted seedlings.In addition,ABA/IAA,ABA/GA3,and ABA/ZR values of grafted leaves were lower than that of ungrafted ones,and ABA/IAA and ABA/GA3 values changed less in grafted seedlings.A significant negative relation between gs and ABA/ZR was detected under copper stress.Response of stoma to copper stress was jointly adjusted by ABA and ABA/ZR to make grafted seedlings keep higher Gs than ungrafted ones,which resulted in higher copper tolerance.【Conclusion】Grafting changed endogenous hormone levels and enhanced resistance against copper stress and effectively maintained the balance of endogenous hormones in plant,which promoted normal growth of melon seedlings. |
| Key words: melon grafting copper stress endogenous hormones |
