| 摘要: |
| 【目的】探讨黄芪、黄芩、大黄、金钱草体外对猪传染性胃肠炎病毒（TGEV）的抑制作用。【方法】采用传统的水提法制备黄芪、黄芩、大黄、金钱草等中药的提取液，测定其对PK-15细胞的最大安全质量浓度。采用细胞病变观察法确定4味中草药对TGEV致PK-15细胞病变的抑制作用，并通过MTT法计算药物的最小抗病毒质量浓度和抑制指数。【结果】黄芪、黄芩、大黄、金钱草的最大安全质量浓度分别为50，12.50，6.25和6.25 mg/mL。黄芩、黄芪能抑制TGEV对PK-15细胞的吸附，抑制指数分别为8和4；大黄、金钱草能直接灭活TGEV病毒，抑制指数均为8。【结论】黄芩、黄芪、大黄、金钱草体外对TGEV均有抑制作用。 |
| 关键词: 中草药 抗病毒 细胞病变 MTT法 |
| DOI： |
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| 基金项目:甘肃省农业科技专项农业科技示范基地建设项目（0623006） |
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| Anti-TGEV effects of four herbs |
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WEI Wen-qiang,WU Run,LIU Lei,et al
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| Abstract: |
| 【Objective】This study investigated the inhibition effects of four herbs (Astragalus,Scutellaria,Rhubarb and Loosestrife) against TGEV in vitro.【Method】The effective components of four herbs were extracted by traditional water-boiling method and their maximum safe concentrations for PK-15 cells were determined.The inhibition activity of four herbs against TGEV infection was determined by cytopathic effect method.The minimal antiviral concentrations and inhibition indexes of the four herbs were tested by MTT method.【Result】The maximum safe concentrations of herbs Astragalus,Scutellaria,Rhubarb and Loosestrife were 50,12.50,6.25,and 6.25 mg/mL,respectively.Scutellaria and Astragalus inhibited the absorption of TGEV on PK-15 cells with the inhibition index of 8 and 4,respectively.Rhubarb and Loosestrife inactivated TGEV directly with the inhibition index of 8.【Conclusion】Astragalus,Scutellaria,Rhubarb and Loosestrife all inhibited TGEV in vitro. |
| Key words: herb antivirus cytopathic effect MTT assay |
