| 摘要: |
| 【目的】研究含银耳多糖的猕猴桃饮料的抗辐射损伤性能，为猕猴桃功能饮料的开发提供参考。【方法】将150只昆明种小鼠随机分为3批，每批50只，再随机分为空白对照组、辐射对照组、低剂量饮料试验组（20.00 mL/kg）、中剂量饮料试验组(40.00 mL/kg)、高剂量饮料试验组(80.00 mL/kg)，每组10只，对各饮料试验组小鼠用含银耳多糖的猕猴桃功能饮料灌胃15 d后，用60Coγ对各组小鼠进行一次性全身照射，观察不同剂量含银耳多糖的猕猴桃功能饮料对小鼠外周血白细胞总数、超氧化物歧化酶（SOD）活性、血清溶血素值的影响效应。【结果】辐照后14 d，辐射对照组、中剂量饮料试验组、高剂量饮料试验组小鼠外周血白细胞计数分别为（5.04±1.39）×109、（9.20±1.93）×109和（11.02±1.08）×109 /L，表明与辐射对照组相比，辐照后的中、高剂量饮料试验组小鼠外周血白细胞总数有显著增加（P<0.05）；辐照后14 d，辐射对照组、中剂量饮料试验组、高剂量饮料试验组小鼠肝脏SOD活性分别为（1 064.52±47.51）、（1 188.56±60.04）和（1 309.54±51.54） U/mg，表明与辐射对照组相比，辐照后的中、高剂量饮料试验组小鼠肝脏SOD活性显著增强（P<0.05）；辐照后18 d，辐射对照组及中、高剂量饮料试验组小鼠血清溶血素值分别为61.40±13.49，89.67±17.95和106.28±13.56，表明与辐射对照组相比，辐照后的中、高剂量饮料试验组小鼠血清溶血素值显著增强（P<0.05）。【结论】饮用40.00和80.00 mL/kg剂量含银耳多糖的猕猴桃饮料对遭受辐射危害的机体具有辅助保护作用。 |
| 关键词: 猕猴桃功能饮料 银耳多糖 抗辐射性能 小鼠试验 |
| DOI： |
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| 基金项目:四川省大学生创新创业训练计划项目（201310619028） |
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| Anti-radiation effect of kiwi beverage containing tremella polysaccharide |
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WANG Yang-yang,XIE Chun,DUAN Xu-fei,et al
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| Abstract: |
| 【Objective】This study investigated the anti-radiation effect of kiwi beverage containing tremella polysaccharide to provide basis for further development of kiwi functional beverage.【Method】150 Kunming mice were randomly divided into three batches with 50 mice in each batch.Each batch included blank control group,radiation control group,low dose group (20.00 mL/kg),middle dose group (40.00 mL/kg),and high dose group (80.00 mL/kg),with 10 mice in each group.After 15 days of gavage with kiwi beverage,the mice were irradiated with 60Co ray for whole body at one-time,then the effects of different tremella polysaccharide doses on total number of peripheral blood leucocytes,activity of superoxide dismutase (SOD),and value of serum hemolysin were observed.【Result】14 days after irradiation,the numbers of peripheral blood leukocytes in the 40.00 mL/kg dose group and 80.00 mL/kg dose group were（9.20±1.93）×109 and （11.02±1.08）×109/L,respectively, significantly higher than the control group of (5.04±1.39)×109/L.The activities of liver super oxide dismutase (SOD) of mice in the control group,40.00 mL/kg dose group and 80.00 mL/kg dose group were（1 064.52±47.51）,（1 188.56±60.04）and (1 309.54±51.54) U/mg,respectively,significantly different from those in the radiation control group.18 days after irradiation,the values of serum hemolysin in the radiation control group,40.00 mL/kg dose group and 80.00 mL/kg dose group were 61.40±13.49,89.67±17.95,and 106.28±13.56,respectively.It shows that mouse serum hemolysin values in middle dose and high dose groups were significantly improved (P<0.05).【Conclusion】Drinking 40.00 or 80.00 mL/kg kiwi beverage containing tremella polysaccharide had auxiliary protection against radiation hazards. |
| Key words: kiwi beverage tremella polysaccharide anti-radiation mice test |
