| 摘要: |
| 【目的】对杀蚊球形芽孢杆菌BS-10高产培养基进行优化，为该菌株的规模化生产提供理论依据。【方法】以菌株BS-10菌体生物量、干质量和芽孢数为考察指标，对培养基中添加的碳、氮组成成分进行正交试验优化，并对优化所得培养基进行发酵罐扩大培养试验和生物活性测定。【结果】菌株BS-10高产毒力最优培养基（NBFB）配方为胰蛋白胨3 g/L，牛肉膏1 g/L，鱼粉6 g/L，麸皮4 g/L；采用NBFB培养基，可将BS-10培养周期缩短至18 h，芽孢数达4.12×109 CFU/mg，对致倦库蚊幼虫的毒力较基础培养基增大了100倍。【结论】菌株BS-10在NBFB培养基扩大培养中，不仅获得了高产量，而且缩短了发酵周期，具有较好的应用潜力和经济价值。 |
| 关键词: 球形芽孢杆菌 菌株BS-10 致倦库蚊 鱼粉 麸皮 |
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| 基金项目:广州市科技局项目（2010J1-E361） |
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| Optimization of high yield medium for Bacillus sphaericus strain 10 |
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LIU Mei,WANG Liu-qing,LIAO Mei-de,et al
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| Abstract: |
| 【Objective】The medium for high mosquito larvicide activity of Bacillus sphaericus strain 10 (BS-10) was optimized to guide large-scale production.【Method】In terms of bacterial biomass,dry weight and spore count of strain BS-10,carbon and nitrogen sources of medium were optimized by orthogonal experiment.Then,the biomass,dissolved oxygen,and other related parameters of optimal medium were evaluated through a 7 L fermentor.【Result】 The optimal medium (NBFB) of BS-10 consisted of 3 g/L tryptone,1 g/L beef extract,6 g/L fish meal and 4 g/L wheat bran.Fermentation period was shortened to 18 h with the optimal medium.The maximum spore count was 4.12×109 CFU/mg and the toxicity against Culex quinquefasciatus was increased by 100 times compared to basal medium (NB medium).【Conclusion】Culturing strain BS-10 in NBFB medium obtained higher larvicide activity and shortened the fermentation period,which has a promising application potential and economic value. |
| Key words: Bacillus sphaericus BS-10 Culex quinquefasciatus fish meal wheat bran |
