| 摘要: |
| 【目的】提高生防枯草芽孢杆菌JN209的稳定性及生防活性。【方法】利用基因工程技术，构建含hrpZPsg12基因的分泌表达载体，采用电转化方法将从大豆细菌性斑点病菌(Pseudomonas syringae pv.glycinea)中克隆到的hrpZPsg12基因转化至枯草芽孢杆菌JN209中，同时采用抑菌圈法测定宿主菌与基因工程菌抑菌活性的变化，评价其作为生物农药在田间对烟草病毒病的生防效果。【结果】成功构建了重组枯草芽孢杆菌基因工程菌JN209/pWB980-hrpZPsg12。抑菌活性分析表明，基因工程菌株在保持原始宿主菌株抑菌活性的同时，对部分靶标菌的抑菌效果有明显提高。烟草病毒病田间小区防治试验表明：与原始宿主菌JN209相比，新构建的基因工程菌JN209/pWB980-hrpZPsg12对烟草病毒病的防效有明显提高。【结论】改良的基因工程菌在抑菌谱和抑菌活性方面均有明显提高，同时对烟草病毒病也有较好的防治效果，可进一步开发为新型生物农药。 |
| 关键词: hrpZPsg12 枯草芽孢杆菌 基因工程菌 抑菌效果 生物农药 |
| DOI： |
| 分类号: |
| 基金项目:吉林省科技发展计划项目（20090211）；吉林农业大学博士科研启动基金项目（201106） |
|
| Construction and biocontrol activity of genetic engineering Bacillus subtilis strain with hrpZPsg12 gene |
|
WANG Xue,TIAN Jia,XU Lin-lin,et al
|
| Abstract: |
| 【Objective】The objective of this study was to improve the stabilities and biocontrol activities of Bacillus subtilis strains JN209.【Method】Bacillus subtilis secretory expression vector was constructed by engineering approach,and hrpZPsg12 from Pseudomonas syringae pv.glycinea was transferred into Bacillus subtilis strain JN209 by electroporation engineering approach.The inhibition activity of JN209 and genetic engineering bacterium JN209/pWB980-hrpZPsg12 against seven plant pathogenic bacteria was determined by inhibition zone method,and the biocontrol effects on tobacco virus diseases was appraised.【Result】Genetic engineering bacterium JN209/pWB980-hrpZPsg12 was successfully constructed.The antibacterial activities against plant pathogenic bacteria were significantly improved in genetic engineering strains.The field trial of controlling tobacco virus disease showed that genetic engineering strains JN209/pWB980-hrpZPsg12 had better control efficiency than the host strain.【Conclusion】Both inhibition spectrum and antibacterial activity were improved significantly in genetic engineering bacteria.This study provides reference for the further development of new biological pesticides. |
| Key words: hrpZPsg12 Bacillus subtilis genetic engineering strain inhibitory effect biological pesticide |
