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不同基因型谷子叶片衰老及活性氧代谢研究
刘凤琴1, 冯强强1, 妙佳源,等1
西北农林科技大学 旱区作物逆境生物学国家重点实验室
摘要:
【目的】探讨谷子叶片衰老的生理机制,为谷子生产与栽培技术研究提供理论依据。【方法】以3个抗旱性不同的谷子品种为材料,研究了谷子开花至成熟期叶片叶绿素含量、可溶性蛋白含量、酶促防御系统保护酶(SOD、CAT、POD)活性及MDA和O2-积累量的动态变化。【结果】谷子开花后功能叶片叶绿素含量以及SOD、POD、CAT活性均在开花7 d达到最大值,其后逐渐下降,各叶位表现为旗叶>倒二叶>倒三叶,不同品种表现为大同32>太选1号>白谷9号;功能叶片可溶性蛋白含量随生育进程的推进逐渐下降,各叶位表现为旗叶>倒二叶>倒三叶,不同品种表现为大同32>太选1号>白谷9号;功能叶片O2-和MDA含量随叶片衰老而增高,各叶位表现为倒三叶>倒二叶>旗叶,不同品种表现为白谷9号>太选1号>大同32;3个谷子品种产量表现为大同32>太选1号>白谷9号。【结论】生产实践中,选择绿叶功能期长、抗逆丰产的品种是旱区谷子丰产的重要因素。
关键词:  谷子  叶片衰老  活性氧代谢
DOI:
分类号:
基金项目:国家自然科学基金项目(31371529);国家谷子糜子产业技术体系项目(CARS-07-12.5-A9);农业部公益性行业(农业)科研专项(200903007);陕西省攻关项目
Leaf senescence and reactive oxygen metabolism of foxtail millet with different genotypes
LIU Feng-qin,FENG Qiang-qiang,MIAO Jia-yuan,et al
Abstract:
【Objective】This study investigated the physiological mechanism for leaf senescence of foxtail millet to provide theoretical basis for production and cultivation of foxtail millet varieties.【Method】Three foxtail millet varieties with different drought resistances were used to explore contents of chlorophyll and soluble protein,activities of enzymatic defense system protective enzymes (SOD,CAT and POD),and dynamic changes of MDA and O2- accumulations in leaves during flowering to ripening stages.【Result】Chlorophyll content and activities of SOD,POD,and CAT reached maximum 7 d after flowering,and then gradually decreased.The performances of leaves were in a decreasing order of flag leaf>top second leaf>top third leaf,while different varieties had a decreasing order of Datong 32>Taixuan 1>Baigu 9.The soluble-protein of different foxtail millet varieties gradually decreased and performances of leaves had the order of flag leaf>top second leaf>top third leaf,while different varieties had the order of Datong 32>Taixuan 1>Baigu 9.O2- and MDA contents increased along with the senescence,performances in leaves had the order of top third leaf>top second leaf>flag leaf,while different varieties performed in the order of Baigu 9>Taixuan 1>Datong 32.Yields of millet varieties were in the decreasing order of Datong 32>Taixuan 1>Baigu 9.【Conclusion】The variety with strong resistance and long lasting green leaf was suggested to improve yield.
Key words:  foxtail millet  leaf senescence  antioxidant metabolism