| 摘要: |
| 【目的】探讨玉米单倍体诱导系JS6-2对不同基础材料、不同授粉时期和果穗不同部位的诱导效果，为玉米单倍体育种技术的应用提供理论依据。【方法】以玉米单倍体诱导系JS6 2为诱导材料，Stock6和农大高诱1号为对照，对不同基础材料进行了杂交诱导，同时比较了不同授粉时期、不同果穗部位对单倍体诱导率的影响。【结果】JS6-2对自交系及杂交种均具有诱导产生单倍体的能力，对自交系诱导中，JS6-2单倍体诱导率（8.29%）明显高于农大高诱1号（1.70%）和Stock6（0.66%）；对杂交种诱导中，JS6-2单倍体诱导率（8.22%）同样明显高于其他2个诱导系（1.35%和0.76%）。JS6-2杂交诱导单倍体最佳时期为雌穗吐丝后3～8 d；果穗顶部的单倍体诱导率（6.87%）高于底部（5.61%）。【结论】玉米单倍体诱导系JS6 2的诱导效果明显好于农大高诱1号和Stock6；Reid、No Reid材料以及Reid×DOM和No Reid×DOM杂优模式材料的单倍体诱导率高；田间杂交诱导时，建议在雌穗吐丝3～5 d整齐剪断花丝进行杂交诱导，单倍体诱导效果好。 |
| 关键词: 玉米 单倍体诱导系 诱导效果 |
| DOI： |
| 分类号: |
| 基金项目:吉林农业大学科研启动基金项目（201204）；国家“863计划”项目（2011AA10A103）；农业部948项目（2011-G1-21）；国家农转资金项目（2014GB2B100006）；吉林省现代农业产业技术体系项目（2013001J-1）；国家转基因重大专项（2014ZX800305B）；国家科技支撑计划项目（2014BAD01B01） |
|
| Induced rate of maize haploid inducer JS6-2 |
|
YANG Wei,REN Xue-jiao,CUI Xue-yu,et al
|
| Abstract: |
| 【Objective】Induction effects of maize haploid inducer JS6-2 with different germplasms,artificial pollination times and sites were studied to improve maize haploid breeding.【Method】Using JS6-2 as inducing line and Stock6 and Cau ho 1 as control,induction rates between inbred lines and hybrids with different artificial pollination times and sites of ear were compared.【Result】JS6-2 had the ability of inducing inbred lines and hybrids.The induction rates of JS6-2 for inbred lines and hybrids were higher than Cau ho 1 and Stock6.The average induction rates for inbred lines were 8.29%,1.70% and 0.66%,while those for hybrids were 8.22%,1.35% and 0.76%,respectively.【Conclusion】JS6-2 was much more efficient than Cau ho 1 and Stock6 in induction of inbred and hybrid lines.Reid,No Reid,Reid×DOM,and No Reid×DOM had high induction rates.Optimal artificial pollination period for inducer JS6-2 was 3-5 d after silking at top site of ear. |
| Key words: maize haploid induce lines effect of induction |
