| 摘要: |
| 【目的】探索葡萄风信子直接再生花芽体系的建立，为葡萄风信子外植体直接再分化花芽研究提供参考。【方法】以葡萄风信子‘亚美尼亚’花蕾外植体为材料，MS为基础培养基，在均添加0.1 mg/L 2,4-D 的条件下，分别用不同质量浓度的6-BA（0，0.5，1，2，3，4 mg/L）、GA3（0，0.5，1，2，3，4 mg/L）和玉米素（0，0.05，0.1，0.5，1，2 mg/L）处理，筛选出诱导花芽的最佳激素及最适质量浓度；以MS+6 BA（或玉米素）2 mg/L+2,4-D 0.1 mg/L研究开花时花瓣切片、花葶、叶片以及叶片诱导的愈伤组织诱导直接再分化花芽的情况。【结果】用MS+2 mg/L 6-BA+0.1 mg/L 2,4-D处理葡萄风信子花蕾时，可在花蕾基部直接再分化出具有蓝色的花芽，且花芽分化率最高，达 60.2%。花葶、花瓣切片、叶片和愈伤组织在同样条件下只能诱导产生愈伤组织、营养芽。说明花蕾是诱导花芽分化的最佳外植体，且最适培养基为MS+2 mg/L 6-BA+0.1 mg/L 2,4-D。【结论】建立了葡萄风信子外植体直接再分化花芽体系。 |
| 关键词: 葡萄风信子‘亚美尼亚’ 直接再分化花芽 营养芽 组织培养 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(31170652) |
|
| Direct regenerated floral and vegetative buds from explants of Muscari armeniacum |
|
LIU Ni-ni,LIU Ya-li,LOU Qian,et al
|
| Abstract: |
| 【Objective】The study investigated the optimal tissue culture conditions for Muscari armeniacum to directly induce the regeneration of floral buds.【Method】Floral buds explants of M.armeniacum were used as materials.With 0.1 mg/L 2,4-D,the buds were treated with different concentrations of 6-BA（0,0.5,1,2,3,4 mg/L）,GA3（0,0.5,1,2,3,4 mg/L）and ZT （0,0.05,0.1,0.5,1,2 mg/L）,respectively to select the optimal induction conditions for bud differentiation.Using MS+2 mg/L 6-BA(or ZT)+0.1 mg/L 2,4-D,effects of different explants including young floral bud,petal segment,pedicel,young leaf and callus on the optimal induction conditions of flower bud were also studied.【Result】The optimal induction conditions for bud differentiation were MS+2 mg/L 6-BA+0.1 mg/L 2,4-D.The optimal induction explant was floral bud with induction rate of 60.2%.With the same hormones conditions,pedicel,sliced petals,leaf and callus explants can only be induced to form callus and vegetative buds.So the optimal induction explant is floral buds,optimal induction condition is MS+2 mg/L 6-BA+0.1 mg/L 2,4-D.【Conclusion】Optimal culture system for M.armeniacum to directly induce the bud differentiation was established. |
| Key words: Muscari armeniacum direct regeneration of floral buds vegetative buds tissue culture |
