| 摘要: |
| 【目的】研究围产期母猪的免疫状态，并初步探讨免疫状态与应激的关系。【方法】在分娩前14 d、分娩当天和分娩后14 d，采集初产母猪血样，检测血清皮质醇、结合珠蛋白及外周血淋巴细胞转化率和嗜中性细胞吞噬能力，采用实时荧光PCR检测外周血嗜中性细胞和淋巴细胞糖皮质激素受体基因(GR)mRNA的表达情况。【结果】分娩当天母猪血清皮质醇和结合珠蛋白的质量浓度分别极显著和显著高于产前14 d和产后14 d的水平。分娩当天母猪外周血嗜中性细胞吞噬率较产前14 d减少40.56%(P＜0.01)，在产后14 d仍未恢复到产前14 d的水平(P＜0.01)。分娩当天母猪外周血淋巴细胞转化率较产前14 d减少40.00%(P＜0.01)，在产后14 d依然维持在较低水平。分娩当天母猪外周血嗜中性细胞GR基因mRNA表达较产前14 d减少43.54%(P＜0.01)，产后14天基本上恢复到产前14 d的水平；外周血淋巴细胞GR基因mRNA表达较产前14 d减少46.67%(P<0.01)，产后14 d依然维持在较低水平。嗜中性细胞和淋巴细胞GR基因mRNA表达与血清皮质醇的水平呈极显著的负相关关系(P＜0.01)。【结论】围产期母猪处于高度应激、健康水平下降和免疫抑制的生理状态。皮质醇可能通过GR途径调节嗜中性细胞和淋巴细胞GR基因mRNA表达，从而影响其功能。 |
| 关键词: 母猪 围产期 应激 免疫状态 |
| DOI： |
| 分类号: |
| 基金项目:河南省重点科技攻关项目（142102110131，112102110123） |
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| Immune status of perinatal sows and the relationship between immune function and cortisol content |
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LIU Yong-xiang,ZHU Kuan-you,LI Wan-tao,et al
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| Abstract: |
| 【Objective】This study aimed to investigate the immune status of perinatal sows and the relationship between the stress status and immune function.【Method】Blood samples of primiparous sows were collected on 14 days before delivery (day -14),the delivery day (day 0),and 14 days after delivery (day +14),and cortisol,haptoglobin,peripheral blood lymphocyte transformation rate,and neutrophil phagocytosis were detected.The mRNA expressions of peripheral blood neutrophil and lymphocyte glucocorticoid receptor were detected by RT-PCR.【Result】The serum cortisol and haptoglobin concentrations on day 0 were significantly and extremely significantly higher than on day -14 and day +14,respectively.The peripheral blood neutrophil phagocytosis on day 0 decreased by 40.56% (P＜0.01) compared to day -14,and it did not recover on day+14 (P＜0.01).The peripheral blood lymphocyte transformation rate on day 0 decreased by 40% compared to day -14 (P＜0.01) and it maintained low on day +14.The neutrophil glucocorticoid receptor (GR) gene mRNA expression on day 0 decreased by 43.54% (P＜0.01) compared to day -14,and it recovered on day +14.The peripheral blood lymphocyte GR gene mRNA expression on day 0 decreased by 46.67% compared to day 0 (P＜0.01) and it remained low on day +14.GR gene expressions of neutrophil and lymphocyte were negatively correlated with serum cortisol concentrations (P＜0.01).【Conclusion】Perinatal sows were in a state of high intensity stress,deteriorated physical health and immunosuppression.Cortisol may regulate neutrophil and lymphocyte GR gene mRNA expressions and affect their functions through GR pathway. |
| Key words: sow perinatal period stress immune status |
