| 摘要: |
| 【目的】研究复方磺胺甲噁唑在鲫鱼体内的药物代谢动力学（以下简称药动学）特征，为制定其合理的用药方案提供理论依据。【方法】对体质量为(68.18±14.68) g/尾的鲫鱼以500 mg/kg的剂量单次口灌复方磺胺甲噁唑粉(100 g含磺胺甲噁唑（SMZ）8.33 g、甲氧苄啶(TMP) 1.67 g)混悬液，并分别于给药后0.25，0.5，0.75，1，1.5，2，4，6，8，12，24，36，48，72 h采集鲫鱼血浆、肌肉、肝和肾4种组织，采用高效液相色谱 紫外检测法(HPLC-UV)对样品进行检测，通过3p97软件分析药动学参数。【结果】甲氧苄啶在鲫鱼血浆、肌肉和肾中的药动学特征符合一级吸收二室开放模型，在肝中的药动学特征符合一级吸收一室开放模型；磺胺甲噁唑在鲫鱼血浆、肌肉、肝和肾中的药动学特征符合一级吸收二室开放模型。【结论】药物的2种主要成分在健康鲫鱼体内吸收较完全，分布广泛，甲氧苄啶较磺胺甲噁唑吸收快、消除慢。 |
| 关键词: 高效液相色谱-紫外检测法 复方磺胺甲噁唑 鲫鱼 药物代谢动力学 |
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| 基金项目:公益性行业(农业)科研专项(201203085)；现代农业产业技术体系建设专项(CARS-46) |
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| Pharmacokinetics of compound sulfamethoxazole in crucian carp |
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HAN Bing,YANG Hong-bo,WANG Di,et al
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| Abstract: |
| 【Objective】This paper studied the pharmacokinetics of compound sulfamethoxazole in crucian carp(Carassius auratus) to provide theoretical basis for designing reasonable medication scheme.【Method】After oral administration of compound sulfamethoxazole (100 g containing 8.33 g SMZ and 1.67 g TMP) suspension liquid with a dosage of 500 mg/kg to crucian carps with weight of (68.18±14.68) g,plasma,muscle,liver and kidney samples were collected at 0.25,0.5,0.75,1,1.5,2,4,6,8,12,24,36,48,and 72 h,respectively.The samples were measured by high performance liquid chromatography-ultraviolet method (HPLC-UV) and the pharmacokinetic parameters were obtained by 3p97 software.【Result】The pharmacokinetics of trimethoprim(TMP) in plasma,muscle,and kidney were in line with the two-compartment open model with first-order absorption and the pharmacokinetics in liver was best described by one-department open model with first-order absorption.The pharmacokinetics of sulfamethoxazole (SMZ) in plasma,muscle,liver and kidney were best described by the two-compartment open model with first-order absorption.【Conclusion】The two main components of compound sulfamethoxazole were well absorbed and widely distributed.TMP was absorbed more rapidly and eliminated more slowly than SMZ. |
| Key words: HPLC-UV compound sulfamethoxazole crucian carp pharmacokinetic |
