| 摘要: |
| 【目的】研究解淀粉芽孢杆菌对番茄灰霉病的防治效果，为该药剂的应用提供参考。【方法】采用菌丝生长速率法和孢子萌发法，分别测定了解淀粉芽孢杆菌（WP）、枯草芽孢杆菌（WP）、40%多菌灵（WP）、50%异菌脲（WP）和40%嘧霉胺（WP）对番茄灰霉病菌菌丝生长和孢子萌发的抑制作用及有效抑制中质量浓度(EC50)；采用盆栽试验和田间试验，分别测定了以上5种药剂对番茄灰霉病的防治效果。【结果】解淀粉芽孢杆菌、枯草芽孢杆菌、40%多菌灵、50%异菌脲和40%嘧霉胺对番茄灰霉病菌菌丝生长抑制的EC50分别为946.28，757.19，75.36，8.95和5.53 μg/mL，对孢子萌发抑制的EC50分别为1 665.56，1 416.16，241.90，37.32和21.35 μg/mL。盆栽试验结果显示，解淀粉芽孢杆菌、枯草芽孢杆菌、40%多菌灵、50%异菌脲和40%嘧霉胺750倍液的保护效果分别为68.47%，70.14%，74.18%，74.13%和80.63%。田间试验结果显示，解淀粉芽孢杆菌、枯草芽孢杆菌、40%多菌灵、50%异菌脲和40%嘧霉胺750倍液的防治效果分别为63.37%，64.83%，69.56%，73.49%和75.55%。【结论】解淀粉芽孢杆菌对番茄灰霉病菌的室内毒力作用和田间防治效果与枯草芽孢杆菌相当，可以作为田间防治番茄灰霉病菌的一种辅助药剂。 |
| 关键词: 解淀粉芽孢杆菌可湿性粉剂 番茄灰霉病 药效试验 辅助药剂 |
| DOI： |
| 分类号: |
| 基金项目:高等学校创新引智计划项目（B07049）；国家“863”计划项目（2007AA021503） |
|
| Toxicity and control efficiency of fungicides against Botrytis cinerea |
|
HUANG Hai,ZHANG Xin,ZOU Hang,et al
|
| Abstract: |
| 【Objective】The toxicity of Bacillus amyloliquefaciens wettable powder (WP) on Bacillus amyloliquefaciens and the control effect on tomato gray mold were studied to improve the application of Bacillus amyloliquefaciens.【Method】The inhibitory effects of five fungicides including 1×109 CFU/g B.amyloliquefaciens WP,1×109 CFU/g B.subtilis WP,40% carbendazim WP,50% iprodione WP and 40% pyrimethanil WP against tomato gray mold pathogen were determined using hyphae growth rate method and spore germination method,and their control effects were determined in pot and field experiments.【Result】The 50% effective concentrations (EC50) of B.amyloliquefaciens,B.subtilis,40% carbendazim,50% iprodione and 40% pyrimethanil against the growth of the hyphae were 946.28,757.19,75.36,8.95 and 5.53 μg/mL,respectively,and their EC50 against the germination of conidia were 1 665.56,1 416.16,241.90,37.32 and 21.35 μg/mL,respectively.The control effects of five fungicides (diluted 750 times solution) were 68.47%,70.14%,74.18%,74.13% and 80.63% in pot experiment and 63.37%,64.83%,69.56%,73.49% and 75.55% in field experiment.【Conclusion】The toxicity and control effect B.amyloliquefaciens WP in field experiment of were equivalent to those of B.subtilis WP.Therefore,B.amyloliquefaciens WP can be used as assistant biofungicides to control tomato gray mold. |
| Key words: Bacillus amyloliquefaciens wettable powder tomato gray mold pryinethanil efficacy trials assistant biofungicides |
