| 摘要: |
| 【目的】损伤挥发物信号能够诱导植物增强对害虫防御的反应，探究3种损伤挥发物信号（绿叶挥发物GLVs、茉莉酸甲酯诱导挥发物 MeJA-VOCs和虫害植物挥发物HIPVs）是否对邻近枣树具有激发作用。【方法】在田间条件下，研究3种损伤挥发物信号（GLVs、MeJA VOCs和HIPVs）对枣树蛋白酶抑制剂（PI）、多酚氧化酶（PPO）和脂氧合酶（LOX）活性的影响，并用GC-MS分析健康枣树、剪叶枣树和虫害枣树挥发物的差异。【结果】3种损伤信号处理均可显著提高（同株）邻近枝或邻近株PI、PPO和LOX活性，表明损伤信号激起了临近枣树的防御反应，启动了枣树的十八烷信号途径。GLVs处理枣树的防御反应可持续48 h，而MeJA-VOCs和HIPVs处理可持续5 d以上。对枣树挥发物的研究发现，剪叶损伤和虫害枣树挥发物组分和含量明显增加，其中枞油烯、乙酸-4-己烯醇酯和3,6,6-三甲基双环［3，1，1］庚-2-烯是枣树损伤后的特异组分，乙酸-4-己烯醇酯是虫害枣树的特异挥发物。【结论】枣树能够识别邻近枣树通过空中传递的损伤信号，提高枣树自身的防御水平。 |
| 关键词: 损伤挥发物 枣树 信号传递 防御反应 激发作用 枣镰翅小卷蛾 |
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| 基金项目:国家“十二五”科技支撑计划项目（2013BAD20B03）；农业部公益性行业专项（201103024） |
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| Transmission and effect of wound signals on defensive response of jujubes |
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FAN Yan-ling,YANG Xiao-ting,LI Xin-gang,et al
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| Abstract: |
| 【Objective】Airborne wound signals could induce plants for enhanced defense against future insect attack,but evidence is sparse in China.The subject of this study was to examine the priming effects of wound signals (GLVs,MeJA-VOCs and HIPVs) on neighboring jujube plants.【Method】Under field conditions,we studied the effects of three damage volatile signals (GLVs,MeJA-VOCs and HIPVs) on jujube protease inhibitor (PI),polyphenol oxidase (PPO) and lipoxygenase (LOX).We also analyzed the differences in volatile organic compounds released by healthy jujube,wound jujube and insect infected jujube using GC-MS.【Result】PI,PPO and LOX activities of adjacent shoots or neighboring plants were significantly enhanced by treatments with these three wound volatiles,indicating that wound signals primed defensive response of neighboring plants or shoots,and activated the octadecanoid signaling pathway.Defense response could last 48 hours in GLVs treated plants,and over 5 days in MeJA-VOCs and HIPVs treated plants.Types and contents volatile components were both remarkably improved in wound plant and insect infected plant.D-Sylvestrene,acetate 4-Hexen-1-ol and 3,6,6-Trimethyl-bicyclo［3,1,1］hept-2-ene were particular constituents after the plants were wounded while acetate 4 Hexen-1-ol was a specific volatile compound in insect infected plants.【Conclusion】Jujube could recognize wound signals released by insect infected plants through airborne transmission and improve their defense levels. |
| Key words: wound signals jujube (Zizyphus jujuba Mill.) signal transmission defensive response priming jujube armyworm (Ancylis sativa Liu) |
