| 摘要: |
| 【目的】通过对华山新麦草与7182远缘杂交获得的抗条锈病新种质系9020-17-25-6进行抗条锈病鉴定和遗传分析，明确9020-17-25-6含有的抗病基因以及细胞学特性。【方法】在温室内以9020-17-25-6、感病对照铭贤169及其杂交后代F1、F2、F3和BC1群体为材料，采用我国目前流行的条锈菌生理小种CYR29、CYR30、CYR31、CYR32和CYR33对供试群体进行苗期抗条锈性鉴定，分析抗病基因的遗传规律，并利用基因组原位杂交(GISH)技术对9020-17-25-6含有的外源染色体片段进行鉴定。【结果】9020-17-25-6在苗期对5个条锈菌生理小种均表现免疫或近免疫，其抗性可能来源于华山新麦草。9020-17-25-6对CYR32和CYR33的抗病性都是由1对显性基因控制。GISH分析表明，9020-17-25-6含有来自华山新麦草的染色体或大的染色体片段，是一个普通小麦-华山新麦草易位系。【结论】华山新麦草易位系9020-17-25-6对我国目前流行的小麦条锈菌生理小种具有良好的抗病性，可以作为抗源在我国小麦抗锈育种中应用。 |
| 关键词: 小麦条锈病 华山新麦草 抗病基因 遗传分析 易位系 原位杂交 |
| DOI： |
| 分类号: |
| 基金项目:公益性行业(农业)科研专项(201203032，201303008)；国家自然科学基金项目(31000846)；湖北省重点(优势)学科作物学(长江大学)资助项目 |
|
| Cytological characterization and resistance inheritance of wheat Psathyrostachys huashanica translocation line 9020-17-25-6 |
|
MA Dong-fang,LIU Shu-yan,FANG Zheng-wu,et al
|
| Abstract: |
| 【Objective】The research was to study the inheritance of resistance to stripe rust in 9020-17-25-6,a wheat Psathyrostachys huashanica translocation germplasm line previously obtained from the interspecific hybridization of wheat genotype 7182 and Psathyrostachys huashanica.【Method】Five different races of Puccinia striiformis f.sp.tritici (Pst),CYR29,CYR30,CYR31,CYR32 and CYR33,were selected to test the F1,F2,F3,and BC1 progenies derived from 9020-17-25-6/Mingxian 169 at seedling stage in greenhouse.Genomic in situ hybridization (GISH) was applied to identify P.huashanica chromosomes in 9020-17-25-6.【Result】9020-17-25-6 was resistant to all tested Chinese races at seedling stage.Genetic analysis revealed that the resistance to CYR32 and CYR33 was controlled by a single dominant gene.A pair of signals was detected when using genomic P.huashanica DNA as a probe in the GISH experiment,and the translocation alien chromatin in this line was very long and cytologically detectable,further indicating that 9020-17-25-6 was a wheat-translocation line.【Conclusion】As a novel resistance resource,9020-17-25-6 had good resistance against popular rusts in China and it can be applied in wheat breeding for rust resistance． |
| Key words: wheat stripe rust Psathyrostachys huashanica resistance genes genetic analysis translocation line GISH |
