| 摘要: |
| 【目的】研究温度和pH对黄金鲈消化酶活力的影响。【方法】采用酶学分析方法，研究不同温度和pH 对黄金鲈蛋白酶、淀粉酶和脂肪酶活力的影响。其中，蛋白酶采用福林 酚法测定，淀粉酶和脂肪酶分别采用淀粉酶试剂盒和脂肪酶试剂盒测定。【结果】在设定的温度和pH 范围内，黄金鲈各组织器官消化酶的活力均随着温度和pH 的升高呈先升高后下降的变化趋势。其中，胃和肝脏蛋白酶的最适温度为 40 ℃，前肠、中肠和幽门盲囊蛋白酶的最适温度均为50 ℃；胃、中肠、肝脏淀粉酶的最适温度均为35 ℃，前肠和幽门盲囊的淀粉酶最适温度为30 ℃；各组织器官脂肪酶活力最适温度均为40 ℃。黄金鲈胃、幽门盲囊、前肠、中肠和肝脏蛋白酶的最适pH分别为2.5，8.0，9.0，9.0和6.0，淀粉酶的最适pH分别为6.0，6.0，7.0，7.0和6.0，脂肪酶的最适pH分别为6.0，7.0，7.0，7.0和7.0。黄金鲈不同组织器官的消化酶活力不同。在最适温度下，不同组织器官蛋白酶活力顺序为幽门盲囊＞前肠＞胃＞中肠＞肝脏，淀粉酶的活力顺序为肝脏＞前肠＞中肠＞幽门盲囊＞胃，脂肪酶的活力顺序为肝脏＞胃＞前肠＞幽门盲囊＞中肠。【结论】黄金鲈消化酶活力的最适温度均高于其生长的最适水温。胃蛋白酶在强酸性时活力较高，肝脏蛋白酶活力在偏酸性条件下较高，肠道和幽门盲囊蛋白酶活力在偏碱性时较高；淀粉酶活力在中性偏酸性时较高；脂肪酶在中性时活力较高。 |
| 关键词: 黄金鲈 消化酶活力 温度 pH |
| DOI： |
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| 基金项目:吉林省科技厅发展计划项目(20110233) |
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| Effects of temperature and pH on activities of digestive enzymes in Perca flavescens |
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YANG Hua,XING Xiu-ping,HUANG Quan,et al
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| Abstract: |
| 【Objective】The aim of the present study was to investigate the effects of temperature and pH on digestive enzymes of Perca flavescens.【Method】Effects of temperature and pH on digestive enzymes of Perca flavescens were studied using enzymatic analysis methods.Forint phenol method was used to determine protease,while amylase and lipase were measured with amylase kit and lipase kit,respectively. 【Result】The activities of all digestive enzymes in each digestive organ of Perca flavescens increased at first and then decreased with the increase of temperature and pH within the designed ranges.In stomach and liver,the optimal temperature for protease was 40 ℃,whereas it was 50 ℃ in anterior intestine,middle intestine and pyloric caeca.The optimum temperature for amylase in stomach,middle intestine and liver was 35 ℃,while was 30 ℃ in other organs.The optimum temperature for lipase in different digestive organs of Perca flavescens was 40 ℃.The optimum pH for protease in stomach,pyloric caeca,anterior intestine,middle intestine and liver were 2.5,8.0,9.0,9.0 and 6.0,respectively.The optimum pH for amylase in stomach,pyloric caeca,anterior intestine,middle intestine and liver were 6.0,6.0,7.0,7.0 and 6.0,respectively.The optimum pH for lipase in stomach was 6.0 while in others were 7.0.The activities of digestive enzymes in Perca flavescens had organ specificity.Under optimum temperature,the activity of protease in different organs was in the order of pyloric caeca＞anterior intestine＞stomach＞middle intestine＞liver,the activity of amylase was in the order of liver＞anterior intestine＞middle intestine＞pyloric caeca＞stomach,and the activity of lipase was in the order of liver＞stomach＞anterior intestine＞pyloric caeca＞middle.【Conclusion】The optimum temperature for digestive enzymes of Perca flavescens was higher than its optimum growth temperature.Pepsin activity was higher in strong acidic conditions,liver protease activity was higher in acidic conditions,and intestinal and pyloric caeca protease activity was higher in alkaline conditions.Amylase activity was higher in slightly acidic conditions and the activity of lipase was higher in neutral conditions. |
| Key words: Perca flavescens activity of digestive enzymes temperature pH |
