| 摘要: |
| 【目的】筛选苹果树腐烂病菌T-DNA插入表型或致病突变体，为研究该病菌的表型及致病相关基因提供起始材料。【方法】从建立的T-DNA插入突变体库中，随机挑选230株突变体菌株进行表型观察及致病性测定，并对部分致病突变体进行TAIL-PCR侧翼扩增。【结果】大部分的突变体与野生型菌株03-8在表型及致病性方面无明显差异，仅30.87%的突变体表现出了明显的变化；其中 16株突变体(占突变体总数的6.95%)致病性发生了明显的增强，而表型与03-8相比无明显的变化；20株突变体(占突变体总数的8.69%)致病性与03-8无显著性差异，但是表型发生了明显的变化；16.1%的突变体则同时表现出表型及致病性的显著差异，其中28株表现为致病性显著降低，7株致病性丧失。通过对15株致病突变体侧翼序列的扩增，获得了7条T-DNA侧翼序列，其中突变体X912的侧翼序列被注释为黄曲霉(Aspergillus flavus)阿魏酸酯酶B前体。【结论】获得了多个不同的苹果树腐烂病菌表型或致病突变体，为研究该病菌的生长发育及致病相关基因奠定了良好的基础。 |
| 关键词: 苹果树腐烂病菌 T-DNA插入 侧翼序列 TAIL-PCR 致病突变体 |
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| 基金项目:国家自然科学基金项目（31171796）；公益性行业（农业）科研专项（201203034）；陕西省科技统筹创新工程计划项目（2011KTZB02-02-02）；高等学校博士学科点专项科研基金项目（20120204110002）；高等学校学科创新引智计划资助项目（B07049） |
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| Phenotype and pathogenicity of Valsa mali T-DNA insertion mutants |
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HUANG Ding-xuan,HU Yang,SUN Guang-chao,et al
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| Abstract: |
| 【Objective】This study aimed to select phenotypic or pathogenic T-DNA insertion mutants of Valsa mali to provide material for further study of phenotype or pathogenicity-related genes.【Method】Two hundred and thirty mutants were randomly selected from the established T-DNA insertion mutant library for phenotypic observation and pathogenicity test.T-DNA flanking sequences of partial pathogenicity mutants were amplified by TAIL-PCR.【Result】Compared to wild-type strain 03-8,most mutants showed no significant difference and only 30.87% mutants exhibited significant changes.Pathogenicity of 16 mutants (6.95%) was significantly enhanced,but there were no significant changes in phenotypes.20 mutants (8.69%) showed significant changes in phenotypes,while no significant difference in pathogenicity.16.1% mutants showed significant changes in both phenotypes and pathogenicity,among which virulence of 28 mutants was significantly reduced and pathogenicity of 7 mutants was completely lost.7 T-DNA flanking sequences were obtained from 15 pathogenicity mutants.The flanking sequence of mutant X912 had high similarity with ferulic acid esterase B precursor of Aspergillus flavus.【Conclusion】Different phenotypic or pathogenic mutants were obtained in this study,which laid foundation for future study of the phenotype and pathogenicity related genes of V.mali. |
| Key words: Valsa mali T-DNA insertion flanking sequences TAIL-PCR pathogenicity |
