| 摘要: |
| 【目的】研究IGF2b基因对建鲤生长及体型的影响。【方法】用含有IGF2b基因的慢病毒载体注射建鲤背部肌肉组织，注射量分别为10，20和40 μL/尾，阴性对照组不进行任何处理，阳性对照组注射20 μL/尾去离子水。在相同饲养条件下饲养满9个月后，分别测量建鲤的各项生长指标。【结果】注射IGF2b基因的建鲤体质量、体长、体高、体厚、尾长均显著大于阴性对照组和阳性对照组，说明IGF2b基因在建鲤背部肌肉组织中过表达对鲤第2阶段生长同样具有促进作用，IGF2b基因在鲤背部肌肉组织中过表达对鲤的体型也有一定的作用。鲤的体长、体高、体厚的3D图也说明，随着IGF2b基因注射，鲤鱼倾向于具有更大的体长、体高、体厚的值。用多元逐步回归法做体质量的回归方程可知，鲤鱼的体质量与体长、体厚、尾长具有线性关系，利用体长、体厚、尾长这3个自变量进行聚类分析，即可准确得到鲤鱼5个注射组间的聚类关系：阴性对照组与阳性对照组共属一类，3个注射组同为一类，其中注射组中10 μL注射组和20 μL注射组为一类，40 μL注射组为一类，IGF2b基因对鲤鱼体型的影响在注射量大于20 μL/尾之后产生了转折，出现了负增长。【结论】IGF2b基因对鲤第2阶段生长同样具有促进作用，且对鲤鱼的体型有一定影响。 |
| 关键词: 建鲤 IGF2b基因 慢病毒载体 不同生长阶段 背部肌肉注射 聚类分析 |
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| 基金项目:中央级公益性科研院所基本科研业务费专项 (2013JBFM14)；“十二五”科技支撑计划项目“大宗淡水主养鱼类新品种选育”(2012BAD26B02)；现代农业产业技术体系建设专项(CARS-46) |
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| Effect of injecting vector containing IGF2b to dorsal muscle tissue on growth of common carp |
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LEI Ying,SU Sheng-yan,DING Jing-bo,et al
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| Abstract: |
| 【Objective】This study investigated the influence of IGF2b gene on growth and body shape of Jian carp.【Method】The lentiviral vector containing IGF2b gene was injected to dorsal muscle of Jian carp with dosages of 10,20,and 40 μL/stern,respectively.The negative control group was not injected and positive control group was injected with 20 μL/stern deionized water.Morphological indexes were measured and compared after 9 months.【Result】Body weight,body length,body height,body thickness,caudal peduncle length,and caudal peduncle thick of carps injected with IGF2b gene were greater than those of the control groups.It was indicated that over expression of IGF2b gene in dorsal muscle of carps improved the growth in the second growth phase and it also had influence on the body shape.3D graph also showed that body weight,body length,and body height of carp were larger as the increase of IGF2b concentration.Multivariate stepwise regression showed that carp weight had a linear relationship with body length,body thickness,and tail handle length.Cluster analysis of three independent variables of body length,body thickness,and tail handle length showed the cluster relationship of the three treatment groups and control groups.Negative control and positive control group clustered together,while all treatment groups clustered as another category.Of the latter category,10 μL injection group and 20 μL injection group belonged to one class,different from the 40 μL injection group.Effect of IGF2b gene on carp shape changed to negative when the injection was greater than 20 μL.【Conclusion】The IGF2b gene promoted the growth of carps in the second growth phase and it also had influence on the body shape of carps. |
| Key words: Cyprinus carpio var Jian IGF2b gene lentiviral vector different growth stages injection in dorsal muscle tissue clustering analysis |
