| 摘要: |
| 【目的】克隆我国重要入侵生物扶桑绵粉蚧（Phenacoccus solenopsis Tinsley）环指蛋白基因（PsRing3）序列，分析其序列特征与表达谱，为进一步揭示扶桑绵粉蚧环指蛋白基因的生理功能提供参考。【方法】用RACE方法克隆扶桑绵粉蚧环指蛋白基因，用生物信息学方法对其编码蛋白进行结构和系统进化分析，用实时荧光PCR方法研究其在各个虫态（卵期及1龄、2龄、3龄若虫和成熟雌虫）中的表达差异。【结果】克隆的扶桑绵粉蚧环指蛋白基因的开放阅读框包含678 bp的片段，编码225个氨基酸。多序列比对表明该蛋白非常保守。系统进化树分析表明，扶桑绵粉蚧与半翅目的豆无网长管蚜（Acyrthosiphon pisum）聚为一支，然后与人体虱（Pediculus humanus corporis）相聚。环指蛋白基因在扶桑绵粉蚧各个虫态均有表达，但以卵期最高，1龄、2龄若虫和成熟雌虫表达量相当。【结论】成功克隆了扶桑绵粉蚧环指蛋白基因，其在不同虫态中的差异表达为进一步揭示该基因在虫体中的生理功能奠定了基础。 |
| 关键词: 扶桑绵粉蚧 环指蛋白 克隆 表达谱 |
| DOI： |
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| 基金项目:农业部公益性行业科研专项（201103026） |
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| Molecular cloning,sequence characteristics and expression of ring finger protein gene of Phenacoccus solenopsis Tinsley |
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LUO Mei,LIN Jin-tian,BIN Shu-ying
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| Abstract: |
| 【Objective】This study cloned the ring finger protein gene of Phenacoccus solenopsis Tinsley (PsRing3),analyzed the sequence features and expression profiles to reveal its physiological function.【Method】The RACE method was used to obtain the full-length PsRing3 gene,the bioinformatics methods were used to analyze the structural features,and the real-time PCR method was used to study the expression profiles of various instars (egg,1st instar larvae,2nd instar larvae,3rd instar larvae and mature females).【Result】We cloned the full length of ring finger protein gene of P.solenopsis Tinsley.The gene contained an open reading frame fragment of 678 bp with 225 amino acids.Multiple sequence alignment indicated that the protein was very conservative.Phylogenetic analysis revealed that PsRing3 was clustered with Acyrthosiphon pisum and Pediculus humanus corporis.Expression of PsRing3 was observed in all instars.The expression in eggs was the highest while expression levels in 1st instar larvae,2nd instar larvae and mature females were equivalent.【Conclusion】The P.solenopsis Tinsley ring finger protein gene was successfully cloned and the differences of its expression in different instars were observed. |
| Key words: Phenacoccus solenopsis Tinsley ring finger protein clone expression profile |
