| 摘要: |
| 【目的】研究24-表油菜素内酯（24-Epibrassinolide，EBR）对酿酒葡萄赤霞珠（Cabernet Sauvignon）和烟73（Yan 73）果实原花色素及黄烷醇含量的影响。【方法】在葡萄转色期之前，使用0.1，0.4，0.8 mg/L 的EBR和1.0 mg/L 油菜素内酯（BRs）抑制剂（Brassinazole，Brz）对酿酒葡萄赤霞珠和烟73果穗进行喷施处理，以喷施清水为对照，在果实成熟过程中测定葡萄果皮和种子中的原花色素及黄烷醇含量，分析不同质量浓度EBR和Brz对葡萄原花色素及黄烷醇含量的影响。【结果】在葡萄成熟过程中，赤霞珠和烟73葡萄果皮中原花色素含量呈现先略有下降然后上升的趋势；赤霞珠果皮中的黄烷醇含量呈现先略有上升后下降然后再上升再下降的趋势，烟73果皮中的黄烷醇含量呈先略有上升后下降然后再上升的趋势。赤霞珠和烟73葡萄种子中的原花色素含量均呈先下降然后上升的趋势；赤霞珠葡萄种子中的黄烷醇含量呈先下降后上升然后再下降的趋势，烟73葡萄种子中的烷醇含量总体呈先下降后上升的趋势。与对照相比，不同质量浓度EBR处理均提高了赤霞珠葡萄果皮与种子中原花色素和黄烷醇的含量，平均提高幅度依次表现为0.4 mg/L EBR＞0.8 mg/L EBR＞0.1 mg/L EBR，其中0.4 mg/L EBR处理与对照之间的差异达显著水平；而不同质量浓度EBR处理均使烟73葡萄果皮与种子中的原花色素和黄烷醇含量有所降低；1.0 mg/L Brz处理降低了赤霞珠和烟73葡萄果皮与种子中的原花色素和黄烷醇含量，其中烟73种子中原花色素和果皮黄烷醇含量与对照之间差异达显著水平。【结论】EBR处理可以提高赤霞珠果皮及种子中的原花色素及黄烷醇含量，但却使烟73 葡萄果皮及种子中的原花色素和黄烷醇含量有所降低；Brz处理降低了赤霞珠和烟73果皮及种子的原花色素和黄烷醇含量，表明外源BRs参与了调节葡萄果皮及种子中原花色素和黄烷醇的积累。 |
| 关键词: 24-表油菜素内酯 原花色素 黄烷醇 酿酒葡萄 |
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| 基金项目:现代农业产业技术体系建设专项（CARS-30）；陕西省自然科学基金项目（2011JM3004） |
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| Effects of 24-Epibrassinolide on contents of proanthocyanidins and flavanol accumulation in wine grape |
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GAO Xiang,XI Zhu-mei,PENG Xiao-qin,et al
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| Abstract: |
| 【Objective】The objective of this study was to investigate the influences of exogenous 24 Epibrassinolide (EBR) treatment on contents of proanthocyanidins and flavanol in Vitis vinifera grape berry (Cabernet Sauvignon and Yan 73).【Method】The grape clusters (Cabernet Sauvignon and Yan 73) were sprayed with 0 (control),0.1,0.4,or 0.8 mg/L EBR and 1.0 mg/L brassinosteroids (BRs) inhibitors (Brassinazole,Brz) before veraison,respectively.The contents of proanthocyanidin and favonol in grape skins and seeds at different periods of fruit ripening were determined to analyze the effects of EBR and Brz on proanthocyanidin and flavanol accumulation.【Result】Proanthocyanidins content had a slight decrease then increased dramatically during fruit ripening in grape skins.On the contrary,flavonol had a gradual increase after treatment,then decreased,and raise again,but decrease again until harvest in Cabernet Sauvignon grape skins,but it had a gradual increase after treatment,then decreased,and raise again in Yan 73 grape skins.Proanthocyandins decreased at first,then increased until harvest in grape seeds,the flavonol had a gradual decrease after treatment,then increased,and decrease again in Cabernet Sauvignon grape seeds,it had a decreased at first,then increased in Yan 73 grape skins.EBR treatment increased the contents of proanthocyanidins and flavanol in grape skins and seeds of Cabernet Sauvignon,and 0.4 mg/L EBR treatment was significantly different from the control.Nevertheless,EBR treatment attenuated the contents of proanthocyanidin and flavanol in grape skins and seeds of Yan 73 during ripening.1.0 mg/L Brz treatment decreased the content of proanthocyanidins and flavanol in both varieties,and the contents of proanthocyanidins in seeds and flavanol in skins of Yan 73 were significantly lower than that of the control.【Conclusion】EBR enhanced the accumulation of proanthocyanidins and flavanol in grape skins and seeds of Cabernet Sauvignon,while the effects on Yan 73 were opposite.Brz treatment decreased the contents of proanthocyanidin and flavanol in grape skins and seeds of these two varieties.The results confirmed that exogenous BRs played a role in accumulation of proanthocyanidin and flavanol in wine grapes. |
| Key words: 24-Epibrassinolide proanthocyanidin flavanol wine grape |
