| 摘要: |
| 【目的】优化蛹虫草子座培养剩余基质中虫草素的提取工艺。【方法】以蛹虫草子座培养剩余基质为材料，采用二次通用旋转组合设计，研究提取温度、提取时间、液料比及提取液pH对虫草素提取得率的影响，建立虫草素提取得率与4个因素间的关系模型，分析其单因子和交互影响效应，通过参数优化得到虫草素的最佳提取工艺条件。【结果】4个因素对虫草素提取得率的影响大小依次为液料比＞提取温度＞提取液pH＞提取时间。在最佳提取工艺条件下，即提取温度为63.3 ℃、提取时间为4.8 h、液料比为36.8、提取液pH为6.7时，虫草素得率可达1.32‰。【结论】获得了从蛹虫草子座培养剩余基质中高效提取虫草素的优化工艺。 |
| 关键词: 蛹虫草 子座培养剩余基质 虫草素 二次通用旋转组合设计 |
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| 基金项目:陕西省科技统筹创新工程计划项目（2011KTCL02-16） |
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| Optimization of the extraction process of cordycepin from Cordyceps militaris culture medium |
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| Abstract: |
| 【Objective】The study was conducted to enhance the extraction yield of cordycepin from Cordyceps militaris culture medium.【Method】Taking C.militaris culture medium as raw material,the quadratic general rotation unitized design method was used to analyze the influence of extraction temperature,extraction time,water material ratio and pH value on cordycepin extraction yield.The model between yield of cordycepin and the four factors was established,single factor and interaction effects were analyzed,and the optimum conditions of extracting cordycepin by optimizing the technological parameters were obtained.【Result】The influences of four studied factors on the extraction yield were in a descending order of water material ratio>extraction temperature>pH value>extraction time.The yield of cordycepin was 1.32‰ when extraction temperature,extraction time,water material ratio and pH value were 63.3 ℃,4.8 h,36.8 and 6.7,respectively.【Conclusion】The technology obtained in this study improved the extraction of cordycepin from C.militaris culture medium. |
| Key words: Cordyceps militaris culture medium cordycepin quadratic general rotation unitized design |
