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不同碳氮源对红汁乳菇菌丝生长的影响
徐鸿雁1, 杜双田1, 孟胜楠1
西北农林科技大学 生命科学学院
摘要:
【目的】探讨不同碳、氮源对红汁乳菇菌丝生长的影响,为红汁乳菇的开发利用提供依据。【方法】以红汁乳菇LH-1菌株为供试材料,以菌落直径、菌丝生长速率和菌丝生长指数为测定指标,研究了13种碳源、22种氮源对红汁乳菇菌丝生长的影响。【结果】红汁乳菇具有较广的碳源及氮源谱,在13种碳源中,以果糖为碳源时,红汁乳菇菌落长势最好,其菌落直径、菌丝生长速率和菌丝生长指数分别为48.31 mm、8.37 mm/d和41.87;在22种氮源中,以酵母膏为氮源时红汁乳菇菌落长势最好,其菌落直径、菌丝生长速率和菌丝生长指数分别为50.82 mm、7.84 mm/d和39.18;红汁乳菇对氨基酸及铵盐类物质利用较差。【结论】红汁乳菇菌丝生长的最佳碳源、氮源分别为果糖和酵母膏。
关键词:  红汁乳菇  碳源  氮源  生长速率
DOI:
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基金项目:陕西省科技统筹创新工程计划项目(2011KTCL02-16)
Effect of different carbon and nitrogen sources on the growth of Lactarius hatsudake Tanaka
Abstract:
【Objective】The research studied the effects of different carbon and nitrogen sources on mycelia growth of Lactarius hatsudake Tanaka.【Method】With L.hatsudake Tanaka LH-1 as the tested materials,the effects of thirteen carbon sources and twenty two nitrogen sources on diameter of colony,mycelia growth rate and the mycelia growth index of L.hatsudake Tanaka were investigated.【Result】L.hatsudake Tanaka had a broader spectrum of carbon and nitrogen sources.Among the thirteen carbon sources,the best was fructose,diameter of colony,mycelia growth rate and the mycelia growth index were 48.31 mm,8.37 mm/d and 41.87,respectively.The best nitrogen sources among the studied twenty two was yeast extract,diameter of colony,mycelia growth rate and the mycelia growth index were respectively 50.82 mm,7.84 mm/d and 39.18,respectively.The utilization of inorganic nitrogen sources and amino acids was poor.【Conclusion】Fructose was identified to be the best carbon source and yeast extract was the best nitrogen source for the mycelia growth of L.hatsudake Tanaka.
Key words:  Lactarius hatsudake Tanaka  carbon sources  nitrogen sources  growth rate