| 摘要: |
| 【目的】探讨棉花黄萎病生防链霉菌25、Z9、Z13、X4对棉花光合生理特性和生长的影响及其在棉花根内的定殖情况。【方法】采用盆栽接种试验、根内微生物培养测数法，以不接链霉菌、大丽轮枝菌（VD）为空白对照，研究4株链霉菌活菌制剂单独接种及与VD混合接种后，链霉菌对棉花幼苗光合生理特性和生长的影响及其在棉花根内的定殖量。【结果】①链霉菌25、Z9、Z13单独接种或与VD混合接种时，均能提高棉花幼苗净光合速率、气孔导度、蒸腾速率。②4株链霉菌均能明显提高棉花幼苗整株和根系鲜质量，其中Z9促生作用最稳定，接种1次后，其单独接种或与VD混合接种处理棉花幼苗根系长度较空白对照、VD单独接种处理增加了56.6%～58.1%，根系鲜质量增加45.9%～54.0%（P＜0.05）。③菌株Z13、25、X4单独接种及与VD混合接种时均可在棉花根内稳定定殖，在菌剂拌土接种后60 d，在棉花根内的定殖量仍可达到(2.0～32.0)×102 cfu/g；Z9接种2次后也能在棉花根内定殖，但定殖量较少。【结论】供试链霉菌能明显提高棉花幼苗光合生理并促进棉花生长，2次接种能促进链霉菌在棉花根内定殖。 |
| 关键词: 生防链霉菌 大丽轮枝菌 定殖 光合生理特性 促生作用 棉花 |
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| 基金项目:国家自然科学基金项目（41261065） |
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| Colonization and effects of antagonistic Streptomyces on leaf photosynthetic characteristics and growth of cotton seedlings |
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| Abstract: |
| 【Objective】This study explored the colonization capability of Verticillium dahliae antagonistic Streptomyces in the root of cotton and effects of the tested Streptomyces on cotton leaf photosynthetic characteristics.【Method】Using pot experiments and traditional cultured based microbiological method,the effects of four strains of Verticillium dahliae antagonistic Streptomyces on cotton growth,leaf photosynthetic characteristics and colonization capability in the root of cotton were studied.Cottons only inoculated VD were the controls.【Result】① Inoculation of Streptomyces 25,Z9,and Z13 individually or mixed with V.dahliae significantly promoted the net photosynthetic rate,transpiration rate,and stomatal conductance.② The fresh plant weights and fresh root weights of cotton seedlings were promoted by all four strains,among which Z9 had the steadiest growth promotion effect on cotton.The cotton root lengths and fresh root weights increased by 56.6%－58.1% and 45.9%－54.0% after one inoculation,respectively with Z9 compared to controls（P＜0.05）.③ Both individual inoculation of or mixed with V.dahliae Z13,25,and X4 steadily colonized in root of cotton.After 60 days of inoculation,the populations were still up to (2.0－32.0)×102 cfu/g in the root of cotton.Z9 could colony in root as well with fewer amounts after double inoculations.【Conclusion】The tested Streptomyces could promote growth and leaf photosynthetic characteristics of cotton.The population of Streptomyces in the root of cotton could increase by double inoculations. |
| Key words: Streptomyces Verticillium dahliae colonization photosynthetic characteristics growth promotion cotton |
