| 摘要: |
| 【目的】优化番茄灰霉病菌拮抗菌的发酵条件，初步探究其抑菌效果，为番茄灰霉病菌的生物防治提供潜在的资源菌。【方法】以前期从大蒜鳞茎中筛选出的拮抗菌D6和D10为研究试材，以接种量、温度、摇床转速和装液量为考察因素，设计四因素三水平正交试验，对D6和D10的发酵条件进行优化，并研究优化条件下D6和D10的无菌滤液对番茄灰霉病菌菌丝生长的抑制作用。【结果】D6和D10的最佳摇床培养条件分别为，D6：接种量4%，温度22 ℃，转速180 r/min，装液量50 mL；D10：接种量4%，温度26 ℃，转速150 r/min，装液量75 mL。当D6的无菌滤液质量分数达到15%时，其抑制率最高达到85.33%；当D10的无菌滤液质量分数达到10%时，其抑制率最高达到70.67%。在121 ℃时，D6无菌滤液的热稳定性较好，而D10的无菌滤液却失去了生物活性。【结论】在优化条件下培养的D6和D10无菌滤液对番茄灰霉病菌有一定的抑制作用，D6的无菌滤液热稳定性较好。 |
| 关键词: 番茄灰霉病菌 拮抗内生菌 无菌滤液 抑菌效果 |
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| 基金项目:陕西省科技统筹创新工程项目(2012CGX7)；陕西省高水平大学建设专项(2012SXTS03) |
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| Optimized fermentation conditions of antagonistic strains D6 and D10 and their inhibitory effect to Botrytis cinerea |
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| Abstract: |
| 【Objective】This study optimized the fermentation conditions of antagonistic strains to Botrytis cinerea,and explored their inhibitory effect preliminarily.【Method】D6 and D10 strains were isolated from the garlic bulb in earlier stage.Inoculum density,culture temperature,shaker revolution and loading volume as investigated factors,4 factors and 3 levels of orthogonal experimental were designed for optimized fermentation conditions of D6 and D10.Their optimum shaker cultivation conditions and germfree fermented filtration inhibitory effect on B.cinerea mycelial growth were investigated.【Result】The obtained optimum shaker cultivation conditions were:D6 inoculum density 4%,culture temperature 22 ℃,shaker revolution 180 r/min and loading volume 50 mL and D10:inoculum density 4%,culture temperature 26 ℃,shaker revolution 150 r/min,and loading volume 75 mL.Highest inhibitory rates were up to 85.33% with the filtrate concentration of 15% and 70.67% with the filtrate concentration of 10%.When the temperature was 121 ℃,the filtration of D6 was stable,while D10 lost biological activity.【Conclusion】Germfree fermented filtrations of D6 and D10 had a striking inhibitory effect to B.cinerea.D6 showed good thermal stability and should be further studied. |
| Key words: Botrytis cinerea endophytic antagonistic bacteria germfree fermented filtrate inhibitory effect |
