| 摘要: |
| 【目的】建立羽衣甘蓝小孢子胚植株再生体系，完善羽衣甘蓝小孢子培养技术，为高效稳定地获得羽衣甘蓝双单倍体植株奠定基础。【方法】以名古屋 红、白珊瑚、红鸥、白舞、桃舞、帝王 桃红、P3、X18和X35等9个基因型羽衣甘蓝为试验材料，研究基因型、培养基、琼脂质量分数、低温处理、胚龄和激素对羽衣甘蓝小孢子胚状体植株再生的影响。【结果】9个基因型羽衣甘蓝的小孢子胚植株成苗率差异很大，其中‘红鸥’的胚状体成苗率最高，达到88.6%；附加质量分数1.0%琼脂的MS培养基最适合植株再生；低温处理对胚状体的再生有一定促进作用，但影响不明显；25～30 d胚龄胚状体的再生能力较强。附加 1.5 mg/L 6-BA和0.2 mg/L NAA 激素的MS培养基有利于胚状体的再生成苗，95%以上的植株能够很好地生根，移栽成活率达75%以上。【结论】对羽衣甘蓝而言，基因型不是其小孢子胚再生能力的惟一决定因素，培养基中有一定量的盐分是小孢子胚再生所必需的条件，小孢子胚萌发成苗时需要相对干燥的环境；在小孢子发育过程中增加脱分化阶段，可使畸形胚、鱼雷型胚或心型胚先形成愈伤组织，再分化形成植株。 |
| 关键词: 羽衣甘蓝 小孢子胚 植株再生 基因型 |
| DOI： |
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| 基金项目:Influencing factors of microspore-derived plant regeneration in ornamental kale (Brassica oleracea var.acephala) |
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| 农业部“948”项目（20032Z36）；武汉市科技计划项目（201250499145-13） |
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| Abstract: |
| 【Objective】Plant regeneration system of microspore-derived embryos was established,and the microspore culture technology was improved to obtain DH (double haploid) plant of ornamental kale efficiently and stably.【Method】Nine genotypes of ornamental kale,such as Red nagoya,White coral,Red gull,White dancing,Prach dancing,Peach dancing,Peach emperor,P3, X18 and X35,were used as experimental material to investigate the effects of genotype,basal medium,agar concentration,cold treatment,embryoid age and PGR on plant regeneration from microspore-derived embryos.【Result】The results showed that there were significant differences in the frequencies of plant regeneration from embryoids among the various genotypes.The cultivar ‘Red gull’achieved the highest frequency of plant regeneration (88.6%).MS medium with 1.0% agar was suitable for plant regeneration.Cold treatment of embryoid could promote the regeneration,but had no significant effect.Embyoid with 25-30 d age showed highest regeneration capacity.The frequency of regeneration was enhanced when embryoids were cultured on medium MS+1.5 mg/L 6-BA+0.2 mg/L NAA.More than 95% showed rooting ability in regenerated plants,among which 75% survived when transplanted.【Conclusion】Genotype was not the only deciding factors for plant regeneration of microspore-derived embryos.It was required for plant regeneration of microspore-derived embryos that culture medium should contain a certain amount of salt.A relatively dry environment was essential for germination of microspore-derived embryos.Cold treatment played an insignificant role in plant regeneration of microspore-derived embryos.The abnormal embryos,torpedo embryo or heart-shaped embryo could dedifferentiate to callus firstly,and then differentiated to plants. |
| Key words: ornamental kale microspore-derived embryos plant regeneration genotype |
