| 摘要: |
| 【目的】研究茉莉酸对棉花幼苗抗虫相关酶活性诱导的浓度依赖性和系统诱导抗性，探讨某一特定浓度茉莉酸诱导抗虫性的滞后期及其对棉铃虫相对生长率和体质量的影响,为农林害虫的综合防治提供理论依据。【方法】以皖棉16 为材料，分别以0.1，1.0 和10 mmol/L茉莉酸处理6 叶期棉花幼苗叶片，研究不同浓度茉莉酸对棉花幼苗抗虫相关酶活性的影响；以1.0 mmol/L茉莉酸处理6叶期棉花幼苗，研究其对棉花幼苗抗虫性诱导的时效性及其对棉铃虫相对生长率和体质量的影响。【结果】0.1 mmol/L 茉莉酸对棉花幼苗多酚氧化酶(PPO)、过氧化物酶(POD）和苯丙氨酸解氨酶(PAL）的诱导作用不明显，但能诱导处理叶脂氧合酶(LOX）活性显著升高；1.0 mmol/L 茉莉酸能诱导棉花幼苗 PPO 和 PAL 活性局部升高和系统升高，但只能诱导 POD 系统升高和 LOX活性局部升高；10 mmol/L 茉莉酸可诱导棉花幼苗 PPO 活性局部升高和系统升高，诱导 PAL 活性系统升高，诱导 LOX 活性系统下降，对 POD 活性的影响不明显。分析表明，1.0 mmol/L 茉莉酸是6 叶期棉花幼苗抗虫相关酶诱导的最佳浓度，诱导效应可持续 4～8 d(依酶而异），但处理后第 6 天的棉叶对棉铃虫相对生长率和体质量增加的抑制作用最明显。【结论】茉莉酸对棉花幼苗抗虫相关酶的诱导存在浓度依赖性和滞后期，诱导抗性明显抑制棉铃虫的相对生长率和体质量增加。1.0 mmol/L 茉莉酸是诱导6叶期皖棉16 幼苗抗虫性的最佳浓度，抗虫性的滞后期可达 4～8 d。 |
| 关键词: 茉莉酸 诱导防御 棉花 棉铃虫 相对生长率 氧化酶 抗消化酶 害虫控制 |
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| 基金项目:国家自然科学基金项目(30970456,31070338)；安徽省自然科学基金项目(090413078,1108085J07)；教育部高等学校博士学科点专项基金项目(新教师类，20093424120002)；生物环境与生态安全安徽省高校省级重点实验室项目 |
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| Resistance of jasmonic acid-mediated cotton seedlings against the relative growth rate of Helicoverpa Armigera |
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| Abstract: |
| 【Objective】The concentration dependency and systemic induction of Wanmian 16(Gossypium hirsutum,L）induced by different concentrations of jasmonic acid(JA）were studied.The time effect of JA application on the relative growth rate and mass gain of Helicoverpa armigera were investigated as well.【Method】0.1,1.0 and 10.0 mmol/L JA were used to investigate their effects on the induction of pest defense enzyme activities in six-foliage seedlings of cotton plant Wanmian 16.1.0 mmol/L JA was used to study the time effect of JA-induced pest defense in cotton seedlings and its effect on the relative growth rate and mass gain of H.armigera.【Result】0.1 mmol/L JA had insignificant effect on the induction of polyphenoxidase(PPO),peroxidase(POD）and phenylalanine ammonia-lyase(PAL）activities,but it induced systemic increase of lipoxygenase(LOX）activity in cotton seedlings.1.0 mmol/L JA induced the local and systemic increase of PPO and PAL activities,but it only induced the systemic increase of POD activity and local increase of LOX activity.10 mmol/L JA induced local and systemic increase of PPO activity,systemic increase of PAL activity and systemic decrease of LOX activity,but its induction effect on POD activity was insignificant.Analysis showed that 1.0 mmol/L JA was the optimal concentration to induce the defense-related enzyme activities.Depending on the enzymes induced,the induced effect could last at least 4-8 days,but the inhibition effect at day 6 was the best in terms of the relative growth rate and mass gain of H.armigera.【Conclusion】JA induced the defense-related enzymes activities in cotton seedlings in a concentration-dependency and time-effect manner.The induced defense by JA inhibited significantly the relative growth rate and mass gain of H.armigera.1.0 mmol/L JA was the optimal JA concentration that induced defense-related enzymes in six-foliage cotton seedlings Wanmian 16,and the duration of induced defense was 4-8 d. |
| Key words: jasmonic acid(JA) induced defense Gossypium hirsutum L. cotton bollworm relative growth rate oxidase antidigestive enzymes pest control |
