| 摘要: |
| 【目的】研究鹿茸对小鼠脑组织抗氧化指标和凋亡相关蛋白表达的影响，为鹿茸的抗氧化和抗凋亡作用研究提供资料。【方法】选用48只6周龄雌性昆明小鼠，随机分为正常组(灌胃生理盐水，n=12)和高、中、低剂量鹿茸冻干粉组(分别灌胃鹿茸冻干粉1,3和5 g/kg，n=12)。各组分别灌胃63 d，第64天处死小鼠并取材。用试剂盒检测小鼠脑组织匀浆液中单胺氧化酶(Monoamine oxidase,MAO)、超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)的活性和丙二醛(Malondialdehyde,MDA)的含量，并采用免疫组化观察小鼠大脑皮层促凋亡蛋白Bax和抗凋亡蛋白Bcl-2的表达情况。【结果】鹿茸冻干粉高剂量组MAO活性极显著低于对照组和中、低剂量组(P＜0.01)，鹿茸冻干粉中剂量组MAO活性极显著低于对照组和低剂量组(P＜0.01)；鹿茸冻干粉高剂量组GSH-Px活性极显著高于对照组和中、低剂量组(P＜0.01)；鹿茸冻干粉高剂量组Bcl-2蛋白表达量极显著高于对照组和中、低剂量组(P＜0.01)，鹿茸冻干粉低剂量组Bcl-2与Bax表达量的比值显著低于对照组和高、中剂量组(P＜0.05)。【结论】高剂量(5 g/kg)鹿茸能提高机体抗氧化酶SOD和GSH-Px活性，降低MAO活性和脂质过氧化物MDA含量，增加抗凋亡蛋白Bcl-2的表达，具有增强机体抵抗生化损伤、清除体内脂质过氧化物、抗凋亡的作用。 |
| 关键词: 鹿茸 免疫组化 单胺氧化酶(MAO) 谷胱甘肽过氧化物酶(GSH-Px) Bax Bcl-2 |
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| 基金项目:农业部“948”项目［2009-Z45(2)］ |
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| Effects of Pilose antler on antioxidation indexes and expression of apoptosis related protein in mice brain |
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| Abstract: |
| 【Objective】This study focused on effects of Pilose antler on antioxidation indexes and protein expression of apoptosis and anti-apoptosis in mice brain.【Method】48 female Kunming mice with age of 6 weeks were randomly divided into 4 groups,control group (intragastric injection of saline,n=12),low-dosage,medium-dosage and high-dosage groups of freeze-drying P.antler (intragastric injection of freeze-drying P.antler 1,3 and 5 g/kg,respectively,n=12 for each group).After being intragastric administrated for 63 days,the mice were killed for measurement of activity of monoamine oxidase (MAO),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and content of malondialdehyde (MDA) on morning of 64th day.Immunohistochemical observation of the cerebral cortex was used to measure the expression of protein Bax and Bcl-2.【Result】The content of MAO in high-dosage group of freeze-drying P.antler was significant lower than that of other groups (P＜0.01).The higher the dosage,the lower the MAO content.The activity of GSH-Px in high-dosage group of freeze-drying P.antler was significant higher than other groups (P＜0.01).Protein expression of Bcl-2 in high-dosage group of freeze-drying P.antler was significant higher than other groups (P＜0.01).The protein expression ratio of Bcl-2 and Bax in low-dosage group of freeze-drying P.antler was significant lower than other groups (P＜0.01).【Conclusion】Statistics showed that high-dosage (5 g/kg) of P.antler can inhibit biochemical damage by promoting activity of SOD and GSH-Px,decreasing activity of MAO,reducing the content of internal lipid peroxide MDA,and increasing anti apoptotic protein expression of Bcl-2. |
| Key words: Pilose antler immunohistochemistry MAO GSH-Px Bax Bcl-2 |
