| 摘要: |
| 【目的】探讨女贞子提取物(Ligustrum lucidum extract)对大强度耐力训练大鼠脑组织的保护作用及其运动能力的影响。【方法】将24只6周龄健康SD大鼠随机分为安静对照组、运动对照组和运动加女贞子提取物组(简称运动加药组)，每组8只，运动对照组进行6周大强度跑台训练，运动加药组除大强度跑台训练外，每天灌服500 mg/L女贞子提取物，给药剂量20 mL/kg，安静对照组和运动对照组灌服同体积Tween-80溶液。6周后将试验大鼠麻醉断髓提取脑组织，测定脑组织总抗氧化能力(T-AOC)，总超氧化物歧化酶(T-SOD)，铜、锌超氧化物歧化酶（Cu，Zn-SOD），锰超氧化物歧化酶（Mn-SOD），谷胱甘肽过氧化物酶(GSH-Px)，过氧化氢酶(CAT)活性和谷胱甘肽(GSH)，丙二醛(MDA)含量。【结果】运动对照组大鼠脑组织T-AOC、T-SOD、Cu，Zn-SOD、Mn-SOD、GSH-Px、CAT活性和GSH含量较安静对照组分别下降21.57%，12.01%，8.43%，19.76%，29.09%，39.62%和19.17%，而MDA含量较安静对照组上升26.15%，运动至力竭时间比安静对照组延长29.01%(P＜0.01)。运动加药组大鼠脑组织T-AOC、T-SOD、Cu，Zn-SOD、Mn-SOD、GSH-Px、CAT活性和GSH含量较运动对照组分别升高20.00%，11.35%，26.46%，33.87%，25.81%，23.61%和19.27%，而MDA含量较运动对照组下降18.04%，运动至力竭时间比运动对照组延长23.09%(P＜0.01)。【结论】补充女贞子提取物可以提高大鼠脑组织中抗氧化酶活性和还原物质含量，防止脑组织氧化损伤，减少MDA生成，对脑组织结构和功能有良好的维护作用，可以提高大鼠运动能力。 |
| 关键词: 女贞子提取物 脑组织 抗氧化酶 氧化损伤 运动能力 大鼠 |
| DOI： |
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| 基金项目:陕西省自然科学基金项目(2003C137) |
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| Effects of Ligustrum lucidum extract on brain tissue oxidative damage of rats taking high-intensity endurance training |
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| Abstract: |
| 【Objective】The study was to explore protection of Ligustrum lucidumextract on the brain tissue damage of rats taking endurance training and its effects on the exercise performance.【Method】24 6-week-old healthy SD rats were randomly divided into quiet control group，exercise control group and exercise drug group.The exercise control group and exercise drug group attended a 6-week high-intensive treadmill exercise.The exercise drug group were also fed with 20 mL/kg weight Ligustrum lucidum extract with concentration of 500 mg/L orally every day.The quiet control group and the exercise control group were fed with same volume of Tween-80 solution.Six weeks later,the rats were anesthetized before their myeloids were broken.The brain tissues were taken and the total antioxidant ability(T-AOC),total superoxide dismutase (T-SOD)，copper,zinc superoxide dismutase (Cu,Zn-SOD)，manganese superoxide dismutase (Mn-SOD)，GSH-Px，CAT activities and GSH，MDA contents were measured.【Result】Compared with the quiet control group,the T-AOC，T-SOD，Cu，Zn-SOD，Mn-SOD，GSH-Px，CAT and GSH activity of the exercise control group decreased by 21.57%，12.01%，8.43%，19.76%，29.09%，39.62% and 19.17%,respectively while the MDA content increased by 26.15%.The exercise exhaustion time of the exercise control groups was 29.01%(P＜0.01) longer than that of the quiet control groups.The T-AOC，T-SOD，Cu，Zn-SOD，Mn-SOD，GSH-Px，CAT and GSH activity of the exercise drug group increased by 20.00%，11.35%，26.46%，33.87%，25.81%，23.61% and 19.27%，respectively,while the MDA content decreased by 18.04% compared to that of exercise control group，the exercise exhaustion time of the exercise drug group increased by 23.09% (P＜0.01).【Conclusion】The Ligustrum lucidum extract could improve brain antioxidant enzyme activities in the drug group,restore the material and prevent oxidative damage in brain tissue.It can reduce MDA formation,maintain brain structure as well.Therefore,it can raise the movement skills of rats. |
| Key words: Ligustrum lucidum extract brain tissue antioxidant enzyme oxidative damage exercise capacity rat |
