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银杏叶提取物对断奶仔猪免疫功能的调节作用
黄其春1,2, 郑新添1,3, 杨小燕1,3
1.龙岩学院 生命科学学院;2.预防兽医学与生物技术福建省高等学校重点实验室;3.福建省人畜寄生与病毒性疫病防控工程技术研究中心
摘要:
【目的】研究银杏叶提取物(EGB)对断奶仔猪免疫功能的调节作用。【方法】将160头25日龄的“杜×长×大”三元杂交断奶仔猪随机分为4组,每组4个重复,每重复10头。第1组为对照组,饲喂基础日粮;第2、3、4组分别饲喂基础日粮中添加质量分数0.1%,0.2%或0.3% EGB的试验日粮。试验期为28 d,分别于第14和28天采集血样,测定断奶仔猪的免疫功能指标。【结果】与对照组相比,日粮中添加质量分数0.2%或0.3% EGB可显著提高断奶仔猪血液红细胞数、淋巴细胞数、血红蛋白质量浓度和淋巴细胞百分率(P<0.05),促进血清免疫球蛋白IgG、IgM、IgA和补体C3、C4的生成以及细胞因子IL-2、IFN-γ的分泌(P<0.05)。【结论】日粮中添加质量分数0.2%或0.3%EGB可增强断奶仔猪的免疫功能。
关键词:  银杏叶提取物  断奶仔猪  免疫功能
DOI:
分类号:
基金项目:福建省科技计划重点项目(2010N0024);福建省高校服务海西建设重点项目(闽教高[2009]8号)
The modulatory role of Ginkgo biloba extract on improving the immune function of weaned piglets
Abstract:
【Objective】The experiment was conducted to investigate the modulatory effects of Ginkgo biloba extract (EGB) on improving the immune function of weaned piglets.【Method】160 weaned crossbred piglets (Duroc×Landrace×Large Yorkshire) with age of 25 days were randomly divided into 4 groups,each with 4 replicates and 10 piglets per replicate.The control group were fed with a basal diet while the other three groups were fed with the same basal diet but supplemented with 0.1%,0.2%,and 0.3% EGB,respectively.The experiment lasted 28 days.On the 14th and 28th day,blood samples were collected and used to represent immune function.【Result】Compared to the control group,adding 0.2% EGB or 0.3% EGB to diets of weaned piglets significantly increased red blood cells,lymphocytes,the contents of hemoglobin and the ratios of lymphocyte.In weaned piglets fed with diets supplemented with 0.2% or 0.3% EGB,the production of serum IgG,IgM,IgA,complement C3 and C4,and the secretion of IL-2,and IFN-γ increased as well (P<0.05).【Conclusion】Adding 0.2% or 0.3% EGB to diets of weaned piglets could enhance their immune function.
Key words:  Ginkgo biloba extract  weaned piglets  immune function