| 摘要: |
| 【目的】研究黄花补血草多糖对小鼠肝癌H22细胞生长的抑制作用及对Bcl-2和Bax蛋白表达的影响。【方法】制备小鼠H22实体瘤模型。提取黄花补血草多糖，研究其对小鼠H22实体瘤的抑瘤率，并通过病理切片观察肿瘤细胞的形态学变化。用免疫组化SP法检测黄花补血草多糖对H22肝癌细胞Bcl-2和Bax蛋白阳性细胞数的影响，探讨其抗肿瘤机理。【结果】以阴性对照组为基数，黄花补血草多糖低（120 mg/kg）、高（240 mg/kg）剂量组及环磷酰胺(CTX，25 mg/kg)阳性对照组实体瘤各组抑瘤率分别为31.32%，40.52%和56.61%，与阴性对照组瘤体质量相比显著或极显著减轻。与阴性对照组相比，黄花补血草多糖低、高剂量组Bcl-2蛋白表达分别显著和极显著下调，而Bax蛋白表达显著上调。黄花补血草多糖高剂量组肿瘤细胞表现出坏死如核固缩深染、碎裂或溶解，甚至出现红染区，个别细胞出现染色质浓缩、细胞皱缩、胞膜空泡化和膜周围凋亡小体的形成等凋亡现象。【结论】黄花补血草多糖对小鼠肝癌H22的生长有一定的抑制作用，其机制可能与抑制Bcl-2和提升Bax蛋白表达，从而促进肿瘤细胞凋亡有关。 |
| 关键词: 黄花补血草 多糖 H22 Bcl-2 Bax |
| DOI： |
| 分类号: |
| 基金项目:中央高校基本科研业务费专项(ZYZ2011068) |
|
| Antitumor effects of Limonium aureum(Linn.) polysaccharides |
|
|
| Abstract: |
| 【Objective】The research was to study the antitumor effects of the stings of polysaccharides from Limonium aureum(Linn.) (LAL-P) on mice bearing hepatocellular carcinoma(H22) and the expression of Bcl-2 and Bax.【Method】The effects of LAL-P on the inhibition rate of solid tumor were studied by building H22 mice tumor model.The morphology changes of tumor cells were observed by pathological section.The effects of expression changes of positive cell of Bcl-2 and Bax protein of LAL-P were observed by immunohistochemical SP method to study its antitumor mechanism.【Result】Compared with the control group,the LAL-P (120 mg/kg and 240 mg/kg) and CTX (25 mg/kg) administrations significantly reduced the tumor weight of solid tumor.The inhibition rates of solid tumor were up to 31.32%,40.52% and 56.61% respectively.There was significant difference compared with the control group.The tumor cells of LAL-P-treated mice showed a massive necrosis such as nucleus atrophy,disintegrating and structureless red staining region.Some cells undergoing apoptosis showed chromatin condensation,cell shrinkage,plasma membrane blebbing and the formation of membrane enclosed apoptotic bodies.Meanwhile,the protein expression of Bax increased and the protein expression of Bcl-2 decreased after oral administration of LAL-P.【Conclusion】LAL-P showed antitumor activity and its mechanism might be related with the up-regulation of Bax and down-regulation of Bcl-2 protein expression. |
| Key words: Limonium aureum(Linn.) polysaccharides H22 Bcl-2 Bax |
