| 摘要: |
| 【目的】探索不同交配时间对小鼠胚胎收集和胚胎发育的影响，为小鼠的超排研究提供参考。【方法】对小鼠进行超数排卵，分别于注射人绒毛膜促性腺激素（hCG）后9，10，11，12，13，14和15 h，在输卵管膨大部收集卵母细胞，研究排卵情况；将超排雌鼠与雄鼠合笼，分别于合笼后2，4，6，8，10和12 h观察小鼠是否见阴道栓，研究小鼠交配时间；为了控制小鼠的交配时间，将超排雌鼠分别于注射hCG后0，2，4，6，8，10，12，14，16和18 h与雄鼠合笼，每个时间点合笼后2 h观察交配情况，于注射hCG后98 h从子宫角收集囊胚，统计胚胎收集率，观察胚胎发育情况。【结果】小鼠排卵主要集中于注射hCG后12～14 h，交配时间主要在注射hCG后的前6 h。人为控制交配时间发现，注射hCG后4，6，8，10和12 h的交配率均较高，较高的胚胎收集率出现在注射hCG后8，10和12 h；囊胚发育率在注射hCG后4 h （56.0%）和8 h（54.5%）较高；扩张囊胚发育率在注射hCG后12 h （68.2%）和16 h （58.0%）显著高于其他时间点（P<0.05）。【结论】交配时间影响小鼠交配成功率、胚胎收集率和胚胎发育；超数排卵后12 h交配可提高小鼠的胚胎收集率和胚胎发育率。 |
| 关键词: 小鼠 超数排卵 交配时间 胚胎收集 胚胎发育 |
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| 基金项目:国家自然科技基金（青年基金）项目（30900155）；陕西省教育厅基金项目(09JK785)；2009年西北大学重点实验室基金项目(200908) |
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| Effect of different mating time on embryonic collection and embryonic development in mice |
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TIAN Jing, ZHAO Gang, ZHONG Gangyi,et al
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College of Life Science,Northwest University
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| Abstract: |
| 【Objective】The effect of different mating time on mice embryonic collection and embryonic development by controlling mating time was investigated.【Method】Ovulation was ensured by observing oocyte in oviduct at 9,10,11,12,13,14,15 h after injecting hCG.To observe the mating time,vaginal plug was recorded at 2,4,6,8,10,12 h after mating of superovulated female with male.To control the mating time,the female was caged with male mice at 0,2,4,6,8,10,12,14,16,18 h after injecting hCG.The mated mice were recorded at 2 h after caging,respectively.The embryo was collected in uterus at 98 h after injecting hCG.The collecting and development rate of embryo were recorded.【Result】In nature,the ovulation occurred at 12-14 h after injecting hCG.The mating rate was mainly concentrated at first 6 h after caging.In control of mating time,mating rate was higher at 4,6,8,10,12 h after injecting hCG.The success of collecting embryo was higher at 8,10,12 h.Formation rate of blastocyst was higher at 4 h (56.0%) and 8 h(54.5%);Development rate of expanded blastocyst was higher at 12 h (68.2%) and 16 h (58.0%),respectively,which were significantly more than other points (P<0.05).【Conclusion】The mating rate,collection and development of embryo was improved by controlling the mating time, and the rates of embryonic collection and development were improved by mating at 12 h after superovulating. |
| Key words: mice superovulation mating time embryonic collection embryonic development |
