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烟草对蚀纹病毒和马铃薯Y病毒的抗病性鉴定
黄 婷1, 袁治礼2, 成巨龙2
1.旱区作物逆境生物学国家重点实验室,农业部西北黄土高原作物有害生物综合治理重点实验室,西北农林科技大学 植物保护学院;2.陕西省烟草研究所
摘要:
【目的】对21份我国生产中大面积推广使用的烟草品种和抗源材料进行烟草蚀纹病毒(TEV)和马铃薯Y病毒(PVY)的抗病性鉴定,为烟草抗病品种的利用与品种合理布局,以及烟草抗病毒病育种的亲本选择提供依据。【方法】以21份烟草品种为材料,于2009-2011年采用大田人工接种鉴定的方法,对21分烟草种质资源进行了TEV和PVY的抗病性鉴定。【结果】在供试种质中,对TEV表现抗病的有双抗70、云烟97、中烟103、秦烟96、辽烟17、云烟203、秦烟98、云烟85、G28共9份材料,表现中抗的有中烟90、龙江237、NC89、G80共4份材料,表现中感的有云烟87、RG11、净叶黄共3份材料,表现感病的有K326、红花大金元、CV87、Coker176、金星6007共5份材料。对PVY表现抗病的材料有2份,分别为辽烟17和金星6007,表现中抗的材料有秦烟98、秦烟96和双抗70共3份材料,表现中感的有NC89、云烟87、净叶黄、G28、云烟85、红花大金元、G80、K326共8份材料,表现感病的有Coker176、龙江237、云烟97、RG11、中烟90、中烟103、CV87、云烟203共8份材料。其中兼抗TEV和PVY 2种病毒病的材料有4份,分别为双抗70、秦烟98、秦烟96和辽烟17;均表现感病的有7份材料,包括Coker176、CV87、红花大金元、K326、净叶黄、RG11和云烟87。TEV和PVY侵染对抗病性较强烟草品种生长发育的影响较小,而对感病品种的影响较大。【结论】不同烟草品种对TEV和PVY的抗病性存在较大差异,抗病性不同的烟草品种在病毒危害以后,病毒对烟叶的产量和叶片生长发育的影响也不同。
关键词:  烟草  烟草蚀纹病毒  马铃薯Y病毒  抗病性鉴定
DOI:
分类号:
基金项目:中国烟草总公司科技重点项目(110200902046);高等学校学科创新引智计划项目(B07049)
Identification of resistance to TEV and PVY in tobacco varieties
Abstract:
【Objective】This study evaluated 21 tobacco germplasm resources for resistance to Tobacco etch virus (TEV) and Potato virus Y (PVY),and the results identified can not only be utilized to provide a basis for selection and variety deployment,but also as parent materials in breeding for resistance.【Method】The identification and evaluation of 21 tobacco varieties resistant to TEV and PVY were carried out by artificial inoculation in the field.【Result】The results showed that the resistance to TEV appeared in 9 varieties,including Shuangkang 70,Yunyan 97,Zhongyan 103,Qinyan 96,Liaoyan 17,Yunyan 203,Qinyan 98,Yunyan 85 and G28;moderate resistance to TEV consisted of 4 varieties,including Zhongyan 90,Longjiang 237,NC89 and G80;moderate susceptible varieties were comprised of Yunyan 87,RG11 and Jingyehuang;susceptible varieties included K326,Honghuadajinyuan,Coker176,Jinxing 6007 and CV87.The resistance to PVY consisted of Liaoyan 17 and Jinxing 6007;moderate resistance to PVY included Qinyan 98,Shuangkang 70 and Qinyan 96;moderate susceptible varieties appeared in 8 varieties,including NC89,Yunyan 87,Jingyehuang,G28,Yunyan 85,G80,K326 and Honghuadajinyuan;susceptible varieties were comprised of Coker176,Longjiang 237,Yunyan 97,RG11,Zhongyan 90,Zhongyan 103,CV87 and Yunyan 203.In this study,4 varieties,including Shuangkang 70,Qinyan 98,Qinyan 96 and Liaoyan 17,were resistant to both virus diseases.7 varieties,including Coker176,CV87,Honghuadajinyuan,K326,Jingyehuang,RG11 and Yunyan 87,were susceptible to both virus diseases.The influence on growth and development of tobacco infected with TEV and PVY was different between disease resistant varieties and the susceptible varieties.【Conclusion】Different tobacco varieties had different disease resistance to TEV and PVY,different resistance of tobacco varieties had different impacts upon output and growth of tobacco leaves.
Key words:  tobacco  tobacco etch virus  potato virus Y  resistance identification