| 摘要: |
| 【目的】研究玉屏风散方剂（以下简称玉方）对施氏鲟(Acipenser schrencki)在适温及热应激条件下生长、生理生化指标、相关非特异性免疫功能的影响。【方法】随机选取体质量0.08～0.10 kg的1+龄施氏鲟，分为试验组和对照组，每组2个重复，每重复15尾鱼，在22 ℃下预饲2周后，试验组鱼按0.9 g/kg的剂量连续口灌给药14 d，分别于试验0，7，10，14 d给药前称量鱼体质量，计算鱼体质量增加率；给药结束后，将试验组和对照组半数鱼放入30 ℃的控温水族箱中热刺激2 h，然后分组采集所有供试鱼的血液及组织样本，进行各种酶活性及活性物质含量的测定。【结果】施氏鲟在玉屏风散口灌给药的10～14 d体质量增加率快速提升。常温给药条件下，玉方总体能明显提高鱼体内球蛋白质量浓度及溶菌酶活性，同时促进AKP活性的提升；而对白细胞吞噬率及吞噬指数无显著影响；且能对SOD活性及NO含量进行平衡调节，并有效降低丙二醛含量。另外，高温热刺激对玉方药效无显著不良影响。但玉方也会在一定程度上导致鱼体血浆中乳酸脱氢酶、谷丙转氨酶活性及总胆红素和尿酸浓度升高。【结论】玉屏风散对促进鱼类生长、增强其免疫及抗氧化能力有一定作用，可用作施氏鲟中药添加剂使用，但使用中应注意该方剂对鱼肝肾功能带来的负担和影响。 |
| 关键词: 玉屏风散 施氏鲟 生理生化 非特异性免疫 |
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| 基金项目:公益性行业（农业）科研专项经费（201003055）；中央级公益性科研院所基本科研业务费专项（201001） |
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| Effect of Jade screen power on the blood biochemical indicators and non specific immunity of Acipenser schrencki |
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| Abstract: |
| 【Objective】In this experiment,effects of Jade screen power on growth,blood biochemical indicators and non specific immunology of one-year-old Amur sturgeon (Acipenser schrencki) in suitable temperature and thermal stimulation were studied.【Method】The 1+old Amur sturgeons with body weight of 0.08-0.10 kg were randomly selected in this study.The experimental group and control group were set up with 15 fish and repeated twice,which were pre-fed for 2 weeks at the temperature of 22 ℃.Two parallel experimental groups were orally given at a dosage of 0.9 g/kg body weight continuously for 14 d and the body weight was measured on 0 d,7 d,10 d and 14 d before the drug administration,respectively,which was used to calculate the weight growth rate (WGR).After the drug administration,half of the fish in the experimental group and control group were put into a temperature controlling aquarium for thermal stimulation at 30 ℃ for 2 h.Then the blood and tissue samples were collected to determine various kinds of enzyme activities and the content of active substances.【Result】The rate of bodyweight gain of Amur sturgeon increased rapidly after oral administration of Jade screen power for 10-14 d.When administrated at the room temperature,Jade screen could improve the globulin (GLU) level and lysozyme (LZM) activity significantly,and promote the increase of AKP content in fish.There was no significant impact of Jade screen on the neutrophil phagocytosis rate and phagocytic index.In addition,Jade screen could balance the SOD activity and NO conten in tissues and decrease effectively the malonaldehyde (MDA) activity.Moreover,no significant harmful impact was found on Jade screen when stimulated at high temperature.However,there were some disadvantages of Jade screen,which could induce the rise of lactate dehydrogenase (LDH) activity,glutamic-pyruvic transaminase (GPT) activity and total bilirubin (TBil) and uric acid (Ua) content in the blood plasma of Amur sturgeon to a certain degree.【Conclusion】All the data indicated that Jade screen power could promote growth,are enhance the immunological and oxidation resistance of the fish.This Chinese medicinal herb compound can be used as a kind of Chinese medicine additive to Amur sturgeon.However,the burden and influence of the medicine to liver and kidney must be noticed when in use. |
| Key words: Jade screen power Amur sturgeon physiology and biochemistry non-specific immunology |
