| 摘要: |
| 【目的】建立猪瘟病毒(CSFV)、高致病性猪蓝耳病病毒(PRRSV)、猪圆环病毒2型(PCV-2)及猪细小病毒(PPV)的基因芯片检测方法，为这4种病的实验室诊断和流行病学调查提供一种快速、高效的病原学诊断方法。【方法】根据GenBank中CSFV、高致病性PRRSV、PCV-2、PPV的相关基因序列，设计合成相关引物，采用PCR方法得到具有荧光标记的PCR产物，然后与含有特定检测探针的芯片进行杂交，建立其基因芯片检测方法，并对该方法的灵敏性和重复性进行检测。应用建立的基因芯片检测方法，对150份临床样品进行检测。【结果】建立了针对CSFV、PRRSV、PCV-2和PPV 4种病毒的基因芯片检测方法，该方法对临床150份样品的检测结果显示，CSFV阳性样品有25份，高致病性PRRSV阳性样品有45份，PCV-2阳性样品有70份，PPV的检测结果全部为阴性，其中混合感染样品有18份。【结论】建立了对CSFV、PRRSV、PCV-2、PPV 4种猪群常见病毒的基因芯片检测方法，该方法具有良好的特异性、敏感性和重复性。 |
| 关键词: 猪病 基因芯片 猪瘟病毒 高致病性猪蓝耳病病毒 猪圆环病毒2型 猪细小病毒 |
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| 基金项目:国家“十一五”科技支撑计划项目（2007BAD86B05） |
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| Development of a genechip for detection of CSFV,PRRSV,PCV-2 and PPV |
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| Abstract: |
| 【Objective】The detection method of CSFV,PRRSV,PCV-2 and PPV is designed by Genechip technique，which is a fast,highly active method for lab diagnosis and epidemiological investigation of the four diseases.【Method】According to the sequence of CSFV,PRRSV,PCV-2 and PPV reported from GenBank,the primers are designed.By PCR,the PCR products are obtained which have fluorescence labeling,then genechip cross,the genechip detection method is established,and its sensitivity and repeatability detected.【Result】The detection method of CSFV,PRRSV,PCV-2 and PPV by Genechip technique is established,and the result of detecting the samples from clinical shows that there are 25 CSFV,45 PRRSV,70 PCV-2,0 PPV,and 18 mixed infections.【Conclusion】The detection method of CSFV,PRRSV,PCV-2 and PPV by Genechip technique is established.The detection method has better specificity,sensitivity and repeatability. |
| Key words: swine disease genechip CSFV PRRSV PCV-2 PPV |
